Ixodidin, a novel antimicrobial peptide from the hemocytes of the cattle tick Boophilus microplus with inhibitory activity against serine proteinases

The presence of an effective immune response in the hemocoel of arthropods is essential for survival as it prevents the invasion of pathogens throughout the animal body. Antimicrobial peptides (AMPs) play an important role in this response by rapidly killing invading microorganisms. In this study, a...

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Bibliographic Details
Published in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2006-04, Vol.27 (4), p.667-674
Main Authors: Fogaça, Andréa C., Almeida, Igor C., Eberlin, Marcos N., Tanaka, Aparecida S., Bulet, Philippe, Daffre, Sirlei
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Anti-Infective Agents - chemistry
Anti-Infective Agents - isolation & purification
Anti-Infective Agents - pharmacology
Antimicrobial Cationic Peptides - chemistry
Antimicrobial Cationic Peptides - isolation & purification
Antimicrobial Cationic Peptides - pharmacology
Antimicrobial peptide
Arthropoda
Biological and medical sciences
Boophilus microplus
Cattle
Escherichia coli
Fundamental and applied biological sciences. Psychology
Hemocyte
Hemocytes - chemistry
Immune system
Ixodidae
Ixodidae - chemistry
Ixodidae - cytology
Micrococcus luteus
Molecular Sequence Data
Peptides - chemistry
Peptides - isolation & purification
Peptides - pharmacology
Sequence Homology, Amino Acid
Serine proteinase inhibitor
Serine Proteinase Inhibitors - chemistry
Serine Proteinase Inhibitors - isolation & purification
Serine Proteinase Inhibitors - pharmacology
Tick
Vertebrates: endocrinology
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Summary:The presence of an effective immune response in the hemocoel of arthropods is essential for survival as it prevents the invasion of pathogens throughout the animal body. Antimicrobial peptides (AMPs) play an important role in this response by rapidly killing invading microorganisms. In this study, a novel cysteine-rich AMP has been isolated and characterized from the hemocytes of the cattle tick, Boophilus microplus. In addition to growth inhibition of Escherichia coli and Micrococcus luteus, the newly described AMP, designated ixodidin (derived from the Family Ixodidae), was found to exert proteolytic inhibitory activity against two exogenous serine proteinases, elastase and chymotrypsin. This is the first report of a molecule of an arachnid that has been shown to inhibit bacterial growth and proteinase activity.
ISSN:0196-9781
1873-5169
DOI:10.1016/j.peptides.2005.07.013