Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma

A murine monoclonal antibody (mAb), CLD3 (IgG1,κ), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tiss...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2005-05, Vol.5 (7), p.1980-1986
Main Authors: Luk, John M., Su, Yvonne C. F., Lam, Stanley C. T., Lee, Carol K., Hu, Mei Yu, He, Qing Yu, Lau, George K., Wong, Fred W. S., Fan, Sheung-Tat
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal - metabolism
Antibodies, Neoplasm - metabolism
Antigens, Nuclear - immunology
Antigens, Nuclear - metabolism
Applied microbiology
Binding Sites, Antibody
Biological and medical sciences
Carcinoma, Hepatocellular - chemistry
Carcinoma, Hepatocellular - immunology
DNA-Binding Proteins - immunology
DNA-Binding Proteins - metabolism
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Gastroenterology. Liver. Pancreas. Abdomen
Hepatocellular carcinoma
Humans
Immunohistochemistry
Ku Autoantigen
Liver Neoplasms - immunology
Liver Neoplasms - metabolism
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Microbiology
Miscellaneous
Molecular Sequence Data
Monoclonal antibody
Proteins
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tumor marker
Tumors
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:A murine monoclonal antibody (mAb), CLD3 (IgG1,κ), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tissues. By immunoprecipitation and using the matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry approach, the antigenic specificity of CLD3 was determined to be heterodimeric Ku70 and Ku80 autoantigen, which was confirmed by Western blotting.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200401084