Identification of a novel cell-adhesive protein spatiotemporally expressed in the basement membrane of mouse developing hair follicle

We used PCR-based cDNA subtraction to screen for genes up-regulated during mouse hair morphogenesis. One gene selected was predominantly expressed at the tip of developing hair follicles and encoded a protein characterized by the presence of twelve tandem repeats of ∼120 amino acids and a novel N-te...

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Bibliographic Details
Published in:Experimental cell research 2005-05, Vol.306 (1), p.9-23
Main Authors: Kiyozumi, Daiji, Osada, Aki, Sugimoto, Nagisa, Weber, Charles N., Ono, Yuichi, Imai, Toshio, Okada, Akiko, Sekiguchi, Kiyotoshi
Format: Article
Language:English
Subjects:
Amino Acid Motifs - genetics
Amino Acid Sequence
Animal Structures - embryology
Animal Structures - metabolism
Animals
Basement membrane
Basement Membrane - embryology
Basement Membrane - metabolism
Binding Sites - genetics
Cell Adhesion - physiology
Chondroitin Sulfate Proteoglycans - genetics
Cloning, Molecular
DNA, Complementary - chemistry
DNA, Complementary - genetics
Extracellular matrix
Extracellular Matrix Proteins - genetics
Extracellular Matrix Proteins - metabolism
Female
Gene Expression Regulation, Developmental
Hair follicle
Hair Follicle - embryology
Hair Follicle - metabolism
HeLa Cells
Humans
In Situ Hybridization
Integrins - genetics
Integrins - metabolism
K562 Cells
Mice
Mice, Inbred ICR
Molecular Sequence Data
Recombinant Proteins - metabolism
RGD
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tandem Repeat Sequences - genetics
Vibrissae - embryology
Vibrissae - metabolism
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Summary:We used PCR-based cDNA subtraction to screen for genes up-regulated during mouse hair morphogenesis. One gene selected was predominantly expressed at the tip of developing hair follicles and encoded a protein characterized by the presence of twelve tandem repeats of ∼120 amino acids and a novel N-terminal domain containing an Arg–Gly–Asp cell-adhesive motif. Immunohistochemistry demonstrated that the protein encoded by this gene, named QBRICK, was localized at the basement membrane zone of embryonic epidermis and hair follicles, in which it was more enriched at the tip rather than the stalk region. Cell adhesion assays showed that QBRICK was active in mediating cell-substratum adhesion through integrins containing αv or α8 chain, but not integrin α5β1. Immunohistochemistry showed that QBRICK colocalized with αv-containing integrins in the interfollicular region, but with the α8-containing integrin at the tip region of developing hair follicles. These results, together, indicate that QBRICK is an adhesive ligand of basement membrane distinctively recognized by cells in the embryonic skin and hair follicles through different types of integrins directed to the Arg–Gly–Asp motif.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2005.01.020