Co‐existence of multiple subclones in TEL‐AML1 at diagnosis of acute lymphoblastic leukaemia in association with submicroscopic deletion of AML1

Summary The TEL/AML1 (ETV6/RUNX1) fusion gene is the most common genetic rearrangement in paediatric acute lymphoblastic leukaemia (ALL). Although considered to be a low‐risk leukaemia, it is associated with a relapse rate of 10–20%. The coexistence of different subclones at diagnosis, based on poly...

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Bibliographic Details
Published in:British journal of haematology 2005-05, Vol.129 (4), p.491-498
Main Authors: Rothman, Rachel, Trakhtenbrot, Luba, Bielorai, Bella, Izraeli, Shai, Ishoev, Galina, Amariglio, Ninette, Rechavi, Gideon, Toren, Amos
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bone Marrow Cells
Child, Preschool
Cloning, Molecular
Core Binding Factor Alpha 2 Subunit
Female
Gene Deletion
Gene Rearrangement
Genetic Markers
Hematologic and hematopoietic diseases
Hematology
Humans
In Situ Hybridization, Fluorescence - methods
Infant
Interphase
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical sciences
multiparametric interphase FISH
Oncogene Proteins, Fusion - genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics
Prognosis
subclones
TEL/AML+ acute lymphoblastic leukaemia
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Summary:Summary The TEL/AML1 (ETV6/RUNX1) fusion gene is the most common genetic rearrangement in paediatric acute lymphoblastic leukaemia (ALL). Although considered to be a low‐risk leukaemia, it is associated with a relapse rate of 10–20%. The coexistence of different subclones at diagnosis, based on polymerase chain reaction (PCR) studies of IG/TCR gene rearrangement, with differential response to chemotherapy, was recently reported in this subtype of ALL. We wished to demonstrate such subclones at diagnosis by a recently developed technique of quantitative multiparametric fluorescence in situ hybridization (FISH). Bone marrow cells from 80 paediatric patients with ALL at diagnosis were analysed for the presence of the TEL/AML1 fusion gene by interphase FISH. Fourteen patients were positive for the translocation. Four of them had several subclones associated with various combinations of additional chromosomal abnormalities. The most striking was an atypical and unexpected hybridization pattern consistent with a submicroscopic deletion of the 5′ region of the AML1 breakpoint. Other abnormalities included TEL deletion, trisomy and tetrasomy 21 as well as double TEL‐AML1 fusion. The presence of numerous subclones in about 25% of patients with TEL/AML1+ ALL suggests extensive clonal evolution by the time of diagnosis.
ISSN:0007-1048
1365-2141
DOI:10.1111/j.1365-2141.2005.05479.x