A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens

A Neisseria gonorrhoeae LightCycler (NGpapLC) assay targeting the porA pseudogene was compared with bacterial culture for detection of N. gonorrhoeae in 636 clinical specimens (216 cervical, 185 urethral, 196 throat, and 39 rectal swab specimens). The specificity of the NGpapLC assay was further inv...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2005-05, Vol.52 (1), p.1-5
Main Authors: Whiley, David M., Buda, Philip P., Freeman, Kevin, Pattle, Neville I., Bates, John, Sloots, Theo P.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Culture Media
DNA, Bacterial - analysis
Female
Fundamental and applied biological sciences. Psychology
Genitalia, Female - microbiology
Genitalia, Male - microbiology
Gonorrhea - diagnosis
Gonorrhea - microbiology
Humans
Infectious diseases
LightCycler
Male
Medical sciences
Microbiology
Miscellaneous
Neisseria gonorrhoeae
Neisseria gonorrhoeae - classification
Neisseria gonorrhoeae - genetics
Neisseria gonorrhoeae - isolation & purification
Polymerase Chain Reaction - methods
Porins - genetics
Pseudogenes
Real-time PCR
Sensitivity and Specificity
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Summary:A Neisseria gonorrhoeae LightCycler (NGpapLC) assay targeting the porA pseudogene was compared with bacterial culture for detection of N. gonorrhoeae in 636 clinical specimens (216 cervical, 185 urethral, 196 throat, and 39 rectal swab specimens). The specificity of the NGpapLC assay was further investigated by testing a bacterial reference panel comprising several Neisseria species. Overall, 19 (3.0%) specimens were positive and 613 (96.4%) specimens were negative by both methods. Four (0.6%) specimens were positive by the NGpapLC assay only. For the cervical and urethral swabs, the NGpapLC provided 100% sensitivity and 100% specificity compared with bacterial culture. Following discrepant analysis, the clinical sensitivity and specificity of the NGpapLC for throat and rectal swabs was also 100%. For the bacterial panel, only N. gonorrhoeae isolates provided positive results. The results show the NGpapLC assay is suitable for use on a range of clinical specimens and could improve detection of pharyngeal N. gonorrhoeae.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2004.12.011