Composition of bone and apatitic biomaterials as revealed by intravital Raman microspectroscopy

Microcharacterization of biominerals allows a better understanding of the pathophysiological events that occur in calcified tissues and synthetic biomaterials. Different methods have been extensively used to conduct such investigations. A new model for the intravital study of the composition and str...

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Bibliographic Details
Published in:Bone (New York, N.Y.) N.Y.), 2005-05, Vol.36 (5), p.893-901
Main Authors: Penel, G., Delfosse, C., Descamps, M., Leroy, G.
Format: Article
Language:English
Subjects:
Animals
Apatites - chemistry
Biocompatible Materials
Biological and medical sciences
Biominerals
Bone
Bone and Bones - chemistry
Female
Fundamental and applied biological sciences. Psychology
Intravital
Protein
Rabbits
Raman
Spectrum Analysis, Raman - methods
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Microcharacterization of biominerals allows a better understanding of the pathophysiological events that occur in calcified tissues and synthetic biomaterials. Different methods have been extensively used to conduct such investigations. A new model for the intravital study of the composition and structure of membranous bone by Raman microspectroscopy is described. Titanium bone chambers equipped with a fused-silica optical window were implanted transcutaneously in the calvaria of New Zealand rabbits. The implanted optical windows were well tolerated, and spectral acquisitions were performed without any additional invasive procedure. Bone and implanted apatitic biomaterials were analyzed at different times after surgery. All Raman bands were unambiguously identified in the bone and biomaterial spectra. The main PO 4 and CO 3 Raman bands in bone spectra were consistent with those found in the carbonated apatite spectrum. The major collagen bands were always observed around 1200–1300 (amide III) and 1600–1700 (amide I) Δcm −1 and, 1400–1470 and 2800–3100 Δcm −1 (bending and stretching modes of CH groups, respectively). The phenylalanine (Phe) band was identified in all spectra at 1003 Δcm −1 and overlapped that of the weak HPO 4 2− ion. The CH bands frequently overlapped the lipid bands. However a distinct protein and lipid bands were detected at 2950 and 2852 Δcm −1, respectively. In bone areas close to blood vessels, the Raman signature of hemoglobin was detected with a characteristic band at 754 Δcm −1. The changes observed in bone varied as a function of time and location. The composition and structure of all of the biomaterials studied—including those that were resorbable—seemed to remain stable over time and location. We report for the first time the complete intravital study of Raman spectra of bone and calcium phosphate biomaterials over a period of 8 months. This new approach does not require specimen preparation and allows simultaneous observation of mineral and organic bone constituents over time, which therefore should provide insightful information about their relationship.
ISSN:8756-3282
1873-2763
DOI:10.1016/j.bone.2005.02.012