The opsonising activity of a pentraxin-like protein isolated from snapper ( Pagrus auratus, Sparidae) serum

In mammals the pentraxins, C-reactive protein (CRP) and serum amyloid P component (SAP) are important components of the immune response. Although pentraxins have been isolated from a number of fish species few studies detail their functional immunological role. In this paper we report the establishm...

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Bibliographic Details
Published in:Developmental & Comparative Immunology 2005, Vol.29 (8), p.703-712
Main Authors: Cook, Mathew T., Hayball, Peter J., Nowak, Barbara F., Hayball, John D.
Format: Article
Language:English
Subjects:
Animals
Azides - pharmacology
C-Reactive Protein - immunology
C-Reactive Protein - isolation & purification
C-Reactive Protein - pharmacology
Fish Proteins - blood
Fish Proteins - immunology
Fish Proteins - isolation & purification
Fish Proteins - pharmacology
Fishes - blood
Fishes - immunology
Flow Cytometry
Fluorescein-5-isothiocyanate - metabolism
Head
Kidney
Leukocytes - cytology
Leukocytes - drug effects
Leukocytes - metabolism
Macrophages
Marine
Opsonin
Opsonin Proteins - immunology
Opsonin Proteins - pharmacology
Pagrus auratus
Pentraxin
Phagocytosis
Phagocytosis - drug effects
Serum Amyloid P-Component - immunology
Serum Amyloid P-Component - isolation & purification
Serum Amyloid P-Component - pharmacology
Snapper
Sparidae
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Summary:In mammals the pentraxins, C-reactive protein (CRP) and serum amyloid P component (SAP) are important components of the immune response. Although pentraxins have been isolated from a number of fish species few studies detail their functional immunological role. In this paper we report the establishment of a flow cytometry based assay to measure the phagocytic activity of isolated snapper head kidney leukocytes (HKLs). This assay was then used to examine the ability of a pentraxin-like protein isolated from the serum of snapper ( P. auratus) (Sn-PLP) to act as an opsonin. Incubation of snapper head kidney leukocytes (HKL) with FITC-labelled beads resulted in uptake of these particles by approximately 35% of HKLs. Incubation of beads with Sn-PLP enhanced phagocytosis by snapper HKLs in a dose-dependant manner. Enhanced phagocytosis could be inhibited by addition of a rabbit anti-Sn-PLP antibody suggesting that Sn-PLP may act as a ligand for a HKL cell surface receptor. This study provides further evidence toward a functional role for pentraxins in the host defence repertoire of fish.
ISSN:0145-305X
1879-0089
1365-2567
DOI:10.1016/j.dci.2004.05.009