Preparation of novel mercury-doped silver nanoparticles film glassy carbon electrode and its application for electrochemical biosensor

A novel mercury-doped silver nanoparticles film glassy carbon (Ag/MFGC) electrode was prepared in this study. Electrochemical behaviors of cysteine on the Ag/MFGC electrode were investigated by electrochemical impedance spectroscopy and cyclic voltammetry (CV). The results indicated that cysteine co...

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Bibliographic Details
Published in:Analytical biochemistry 2005-06, Vol.341 (1), p.52-57
Main Authors: Li, Mao-Guo, Shang, Yong-Jia, Gao, Ying-Chun, Wang, Guang-Feng, Fang, Bin
Format: Article
Language:English
Subjects:
Adsorption
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensor
Carbon
Cysteine
Cysteine - chemistry
Electrochemical behavior
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Mercury
Mercury-doped silver nanoparticles
Nanostructures
Silver
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Summary:A novel mercury-doped silver nanoparticles film glassy carbon (Ag/MFGC) electrode was prepared in this study. Electrochemical behaviors of cysteine on the Ag/MFGC electrode were investigated by electrochemical impedance spectroscopy and cyclic voltammetry (CV). The results indicated that cysteine could be strongly adsorbed on the surface of the Ag/MFGC electrode to form a thin layer. The doped electrode could catalyze the electrode reaction process of cysteine, and the cysteine displayed a pair of well-defined and nearly reversible CV peaks at the electrode in an acetate buffer solution (pH 5.0). The Ag/MFGC electrode was used for determination of cysteine by differential pulse voltammetry. The linear range was between 4.0 × 10 −7 and 1.3 × 10 −5 mol/L, with a detection limit of 1.0 × 10 −7 mol/L and a signal-to-noise ratio of 3. The relative standard deviation was 2.4% for seven successive determinations of 1.0 × 10 −5 mol/L cysteine. The determinations of cysteine in synthetic samples and urinal samples were carried out and satisfactory results were obtained. Amperometric application of the Ag/MFGC electrode as biosensors is proposed.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2005.02.014