Differential display of proteins involved in the neural differentiation of mouse embryonic carcinoma P19 cells by comparative proteomic analysis

Mouse embryonic carcinoma P19 cell has been used extensively as a model to study molecular mechanisms of neural differentiation in vitro. After retinoic acid (RA) treatment and aggregation, P19 cells can differentiate into neural cells including neurons and glial cells. In this study, comparative pr...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2005-04, Vol.5 (6), p.1656-1668
Main Authors: An, Jie, Yuan, Quan, Wang, Chen, Liu, Li, Tang, Ke, Tian, Hong-yu, Jing, Nai-he, Zhao, Fu-kun
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cell Differentiation
Cell Line, Tumor
Comparative proteomics
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Mass spectrometry
Mice
Miscellaneous
Neural differentiation
Neuroglia - cytology
Neuroglia - metabolism
Neurons - cytology
Neurons - metabolism
P19 cell
Proteins
Proteome - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tretinoin - pharmacology
Two-dimensional gel electrophoresis
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Summary:Mouse embryonic carcinoma P19 cell has been used extensively as a model to study molecular mechanisms of neural differentiation in vitro. After retinoic acid (RA) treatment and aggregation, P19 cells can differentiate into neural cells including neurons and glial cells. In this study, comparative proteomic analysis is utilized to approach the protein profiles associated with the RA‐induced neural differentiation of P19 cells. Image analysis of silver stained two‐dimensional gels indicated that 28 protein spots had significantly differential expression patterns in both quantity and quality. With mass spectrometry analysis and protein functional exploration, many proteins demonstrated an association with distinct aspects of neural differentiation. These proteins were gag polyprotein, rod cGMP‐specific 3',5'‐cyclic phosphodiesterase, 53 kDa BRG1‐associated factor A, N‐myc downstream regulated 1, Vitamin D receptor associated factor 1, stromal cell derived factor receptor 1, phosphoglycerate mutase, Ran‐specific GTPase‐activating protein, and retinoic acid (RA)‐binding protein. While some cytoskeleton‐related proteins such as beta cytoskeletal actin, gamma‐actin, actin‐related protein 1, tropomyosin 1, and cofilin 1 are related to cell migration and aggregation, other proteins have shown a relationship with distinct aspects of neural differentiation including energy production and utilization, protein synthesis and folding, cell signaling transduction, and self‐protection. The differential expression patterns of these 28 proteins indicate their different roles during the neural differentiation of P19 cells. As an initial step toward unveiling the regulations involved in the commitment of pluripotent cells to a neural fate, information from this study may be helpful to uncover the molecular mechanisms of neural differentiation.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200401049