Human Leukocyte Antigen-G, a New Parameter in the Follow-up of Liver Transplantation

Human leukocyte antigen-G (HLA-G) displays immunotolerogenic properties toward effector cells in graft rejection through inhibition of natural killer (NK) and cytotoxic T lymphocyte (CTL)-mediated cytolysis and CD4+ T-cell alloproliferation. CD4(+)CD25(+) high regulatory T (Treg) cells are pivotal f...

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Bibliographic Details
Published in:Transplantation proceedings 2006-03, Vol.38 (2), p.571-574
Main Authors: Baştürk, B., Karakayalı, F., Emiroğlu, R., Sözer, O., Haberal, A., Bal, D., Haberal, M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood Donors
Cells, Cultured
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histocompatibility Antigens Class I - blood
HLA Antigens - blood
HLA-G Antigens
Humans
Interleukin-10 - biosynthesis
Liver Function Tests
Liver Transplantation - immunology
Medical sciences
Postoperative Period
Prospective Studies
Reference Values
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
Tissue, organ and graft immunology
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Summary:Human leukocyte antigen-G (HLA-G) displays immunotolerogenic properties toward effector cells in graft rejection through inhibition of natural killer (NK) and cytotoxic T lymphocyte (CTL)-mediated cytolysis and CD4+ T-cell alloproliferation. CD4(+)CD25(+) high regulatory T (Treg) cells are pivotal for the maintenance of self-tolerance of pathogenic alloresponses after solid organ or bone marrow transplantation in murine model systems. The aim of this study was to investigate whether there was an association between soluble and membrane-bound HLA-G levels on Treg cells and liver graft prognosis. For this purpose, we studied 37 liver transplant patients and 13 healthy blood donors. To investigate the expression of HLA-G on the surface of peripheral mononuclear (PMNL) cells, we have used monoclonal antibodies in flow cytometry to estimate CD4, CD25, CD45, and HLA-G content. HLA-G serum levels were determined by ELISA. We observed a correlation between sHLA-G serum levels and liver function tests. After a month of HLA-G decrease in serum levels, liver function tests such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), direct bilirubin (DB), total bilirubin (TB), and alkaline phosphatase (ALP) were above normal levels, suggesting liver dysfunction or rejection. Considering these results, we concluded that the increased sHLA-G in serum and on cell surfaces may afford preliminary data on the prognosis and response to treatment in liver transplant patients.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2005.12.108