High salinity reduces the content of a highly abundant 23-kDa protein of the mangrove Bruguiera parviflora

A significant decrease in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular mass of 23 kDa and pI = 6.5, was observed in leaves of NaCl-treated Bruguiera parviflora (Roxb.) Wt. & Arn. ex Griff. seedlings. This particular salt-sen...

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Bibliographic Details
Published in:Planta 2005-04, Vol.221 (1), p.135-140
Main Authors: Parida, Asish Kumar, Mittra, Bhabatosh, Das, Anath Bandhu, Das, Taposh Kumar, Mohanty, Prasanna
Format: Article
Language:English
Subjects:
Antibodies
Biological and medical sciences
Blotting, Western
Cell biochemistry
Cell physiology
Chloroplasts
Chloroplasts - chemistry
Chloroplasts - metabolism
Cytosol
Electrophoresis, Polyacrylamide Gel
Enzymes
Fundamental and applied biological sciences. Psychology
Gels
Leaves
Molecular Weight
Osmolar Concentration
Plant Leaves - drug effects
Plant Leaves - metabolism
Plant physiology and development
Plant Proteins - isolation & purification
Plant Proteins - metabolism
Plants
Proteins
Rhizophoraceae - drug effects
Rhizophoraceae - metabolism
Salts
Seedlings
Sodium Chloride - pharmacology
Table salt
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Summary:A significant decrease in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular mass of 23 kDa and pI = 6.5, was observed in leaves of NaCl-treated Bruguiera parviflora (Roxb.) Wt. & Arn. ex Griff. seedlings. This particular salt-sensitive protein, designated as SSP-23, almost disappeared after 45 days of treatment in 400 mM NaCl as compared to untreated seedlings (0 mM NaCl) where the presence of the protein was significant. A polyclonal antibody raised against the 23-kDa protein was used to determine the subcellular localization of this protein in leaves by cross-reaction with proteins from isolated chloroplasts, mitochondria, peroxisomes and cytosol fractions on Western blots. SSP-23 was confirmed to be localized in the cytosol by immunoblotting. The disappearance of SSP-23 as a result of high NaCl treatment suggests that this protein is salt-sensitive and has a possible role in salt adaptation.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-004-1415-2