Enhanced clinical utility of the NucliSens EasyQ RSV A+B Assay for rapid detection of respiratory syncytial virus in clinical samples

The aim of the present study was to compare traditional methods for the detection of respiratory syncytial virus with a newly developed commercial assay based on real-time nucleic acid sequence based amplification. Respiratory syncytial virus is a major cause of severe respiratory infection in infan...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2006-03, Vol.25 (3), p.167-174
Main Authors: MOORE, C, VALAPPIL, M, CORDEN, S, WESTMORELAND, D
Format: Article
Language:English
Subjects:
Adult
Aged
Animals
Biological and medical sciences
Cell Line
Child
Child, Preschool
Female
Human viral diseases
Humans
Infant
Infectious diseases
Male
Medical sciences
Middle Aged
Reagent Kits, Diagnostic
Respiratory Syncytial Virus Infections - diagnosis
Respiratory Syncytial Virus Infections - virology
Respiratory Syncytial Virus, Human - genetics
Respiratory Syncytial Virus, Human - isolation & purification
RNA, Viral - analysis
Self-Sustained Sequence Replication - methods
Time Factors
Viral diseases
Viral diseases of the respiratory system and ent viral diseases
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Summary:The aim of the present study was to compare traditional methods for the detection of respiratory syncytial virus with a newly developed commercial assay based on real-time nucleic acid sequence based amplification. Respiratory syncytial virus is a major cause of severe respiratory infection in infants and in certain groups of older children and adults. Treatment options are limited, but a rapid diagnosis improves patient management and infection control. The rapid diagnosis of respiratory syncytial virus currently relies on antigen detection assays. These tests are limited to use in certain good-quality types of samples, which are rarely obtained from adult patients. Molecular-based assays for the detection of respiratory syncytial virus are shown to be highly sensitive, specific, and more rapid than cell culture techniques. This retrospective study compared traditional laboratory techniques for the detection of respiratory syncytial virus in 508 respiratory samples collected during the winter months of 2003-2004 against the recently developed, commercially available NucliSens EasyQ Respiratory Syncytial Virus A+B assay (bioMérieux, Marcy l'Etoile, France), which is based on real-time nucleic acid sequence based amplification using molecular beacons and an internal control. Using traditional techniques, the prevalence of respiratory syncytial virus in the samples tested was found to be 21%. Using the real-time nucleic acid sequence-based amplification assay, an additional 41 samples from patients with a clinically diagnosed respiratory illness were found to be positive for respiratory syncytial virus. The NucliSens EasyQ assay was shown to be sensitive and specific for the detection of respiratory syncytial virus A+B in different types of respiratory samples. Moreover, the time required to complete the assay was
ISSN:0934-9723
1435-4373
DOI:10.1007/S10096-006-0112-4