Proteomic analysis of differential protein expression in human atherosclerotic plaque progression

In this study, differential protein expression was assessed during human atherosclerotic plaque progression. A multifaceted approach was used in which differential protein expression was studied by two‐dimensional (2D) gel electrophoresis and validated in individual patients using western blotting a...

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Bibliographic Details
Published in:The Journal of pathology 2005-05, Vol.206 (1), p.39-45
Main Authors: Donners, Marjo MPC, Verluyten, Monique J, Bouwman, Freek G, Mariman, Edwin CM, Devreese, Bart, Vanrobaeys, Frank, van Beeumen, Jozef, van den Akker, Luc HJM, Daemen, Mat JAP, Heeneman, Sylvia
Format: Article
Language:English
Subjects:
2D gel electrophoresis
Adaptor Proteins, Signal Transducing - genetics
Adaptor Proteins, Signal Transducing - metabolism
Aged
alpha 1-Antitrypsin - genetics
alpha 1-Antitrypsin - metabolism
Arteriosclerosis - immunology
Arteriosclerosis - metabolism
atherosclerosis
Disease Progression
Electrophoresis, Gel, Two-Dimensional
Endothelial Cells - metabolism
Gene Expression
human
Humans
Immunohistochemistry
Macrophages - metabolism
Male
Mass Spectrometry
Middle Aged
Muscle Proteins - genetics
Muscle Proteins - metabolism
Neutrophils - metabolism
Proteomics
Thrombosis - immunology
Thrombosis - metabolism
vinexin-β
α1-antitrypsin
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Summary:In this study, differential protein expression was assessed during human atherosclerotic plaque progression. A multifaceted approach was used in which differential protein expression was studied by two‐dimensional (2D) gel electrophoresis and validated in individual patients using western blotting and immunohistochemistry. 2D profiles of whole‐mount advanced stable lesions were compared to those of plaques containing a thrombus. Mass spectrometry analysis identified vinexin‐β and α1‐antitrypsin (AAT) in the same spot that was differentially expressed in plaques with a thrombus. Immunohistochemistry and western blotting showed limited expression of both vinexin‐β and AAT in early lesions, whereas high expression of both proteins was found in advanced lesions. Differential expression of vinexin‐β in lesions with a thrombus compared to stable plaques could not be confirmed, indicating the importance of validation of proteomic analysis. For AAT, western blotting of 2D gels revealed expression of six isoforms in advanced plaques, one of which was confirmed to be solely expressed in thrombus‐containing plaques. In conclusion, vinexin‐β is expressed in advanced human atherosclerotic plaques, but differential expression of this protein in lesions with a thrombus versus stable plaques could not be confirmed. However, this analysis revealed expression of six isoforms of AAT in advanced plaques, one of which was uniquely expressed in thrombus‐containing plaques. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
ISSN:0022-3417
1096-9896
DOI:10.1002/path.1749