Chicken embryo related (CER) cell line for quantification of rabies neutralizing antibody by fluorescent focus inhibition test

Levels of rabies virus neutralization antibody in sera from vaccinated dogs and cattle were either measured by mouse neutralization test (MNT) or by rapid fluorescent focus inhibition test (RFFIT), performed on CER monolayers. The two tests were compared for their ability to detect the 0.5 Internati...

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Bibliographic Details
Published in:Biologicals 2006-03, Vol.34 (1), p.29-32
Main Authors: Cardoso, T.C., da Silva, L.H. Queiroz, da Silva, S.E.L., Albas, A., Pardo, P.E., Tanaka, A.H., Cossy, L.B., Perri, S.H.V.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
BHK-21 cells
Cattle
Cell Line
CER (chicken embryo related) cells
Chick Embryo - cytology
Dogs
Fluorescence
Fluorescent Antibody Technique, Indirect - methods
Mice
Neutralization Tests
Rabies
Rabies - diagnosis
Rabies virus
Rabies virus - immunology
Rapid focus fluorescent inhibition test (RFFIT)
Sensitivity and Specificity
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Summary:Levels of rabies virus neutralization antibody in sera from vaccinated dogs and cattle were either measured by mouse neutralization test (MNT) or by rapid fluorescent focus inhibition test (RFFIT), performed on CER monolayers. The two tests were compared for their ability to detect the 0.5 International Units/ml (I.U.) recommended by the World Health Organization (WHO) as the minimum response for proof of rabies immunization. A significant correlation was found between the two tests ( n = 211; r = 0.9949 in dogs and 0.9307 in cows, p < 0.001), good sensitivity (87.5%), specificity (94.7%) and agreement (96.6%) as well. RFFIT method standardized on CER cell system for neutralizing antibodies detection turns the diagnosis easier and less expensive, specially when a great number of samples must be tested from endemic areas as commonly found in Brazil.
ISSN:1045-1056
1095-8320
DOI:10.1016/j.biologicals.2005.08.001