Role of Caveolin-1 and Cholesterol in Transmembrane Fatty Acid Movement

We have created by transfection a series of HEK 293 cell lines that express varying amounts of caveolin-1 to test the possible effect of this protein on the transport and metabolism of long chain fatty acids (FA) in cells with this gain of function. We used an extracellular fluorescent probe (ADIFAB...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Easton) 2006-03, Vol.45 (9), p.2882-2893
Main Authors: Meshulam, Tova, Simard, Jeffrey R., Wharton, Jonathan, Hamilton, James A., Pilch, Paul F.
Format: Article
Language:English
Subjects:
Animals
Biological Transport - drug effects
Caveolin 1 - metabolism
Caveolin 1 - pharmacology
Cell Line
Cell Membrane - metabolism
Cholesterol - metabolism
Cholesterol - pharmacology
Fatty Acids - metabolism
Humans
Mice
Models, Biological
Time Factors
Transfection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have created by transfection a series of HEK 293 cell lines that express varying amounts of caveolin-1 to test the possible effect of this protein on the transport and metabolism of long chain fatty acids (FA) in cells with this gain of function. We used an extracellular fluorescent probe (ADIFAB) to monitor binding of exogenous FA to the plasma membrane and an intracellular pH probe to monitor FA equilibration across the plasma membrane. Real-time fluorescence measurements showed rapid binding of oleic acid to the extracellular side of the plasma membrane and a rapid translocation across the lipid bilayer by the flip-flop mechanism (
ISSN:0006-2960
1520-4995
DOI:10.1021/bi051999b