Crosslinking of phenylboronic acid receptors as a means of glucose selective holographic detection

A holographic sensor for the detection of glucose has been developed that is based on a hydrogel film containing phenylboronic acid receptors. Changes to the replay wavelength of the hologram were used to characterise the swelling and de-swelling behaviour of the hydrogel matrix upon receptor-ligand...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2006-03, Vol.21 (9), p.1838-1845
Main Authors: Horgan, Adrian M., Marshall, Alexander J., Kew, Simon J., Dean, Kathryn E.S., Creasey, Chris D., Kabilan, Satyamoorthy
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Boronic acid
Boronic Acids - analysis
Boronic Acids - chemistry
Cross-Linking Reagents
Diabetes
Fundamental and applied biological sciences. Psychology
Glucose
Glucose - analysis
Glucose - chemistry
Hologram
Holography - methods
Methods. Procedures. Technologies
Refractometry - methods
Reproducibility of Results
Sensitivity and Specificity
Sensor
Synthetic receptor
Various methods and equipments
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Summary:A holographic sensor for the detection of glucose has been developed that is based on a hydrogel film containing phenylboronic acid receptors. Changes to the replay wavelength of the hologram were used to characterise the swelling and de-swelling behaviour of the hydrogel matrix upon receptor-ligand binding. The effect of introducing a fixed positive charge into the polymer matrix by modification of the hydrogel with a quaternary amine group (3-acrylamidopropyl)trimethylammonium chloride (ATMA), was investigated for a range of sugars and the α-hydroxy acid, lactate, at physiological pH. The quaternary amine-modified hydrogel matrix was found to contract in the presence of glucose, whereas, it was minimally responsive to other saccharides. The selectivity of the sensor for glucose compared to lactate was also significantly improved compared to the unmodified film. A crosslinking mechanism is proposed to explain the enhanced selectivity to glucose.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2005.11.028