A comparative study of secretory immunoglobulin A and immunoglobulin G in host defense in an in vitro pneumonia model

An exaggerated inflammatory response to infections including nosocomial pneumonia may be detrimental to the host and contribute to morbidity and mortality. Both secretory immunoglobulin A (SIgA) and IgG contribute to the immune defense of respiratory surfaces. However, their relative ability to prot...

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Bibliographic Details
Published in:The American journal of surgery 2006-03, Vol.191 (3), p.386-390
Main Authors: Diebel, Lawrence N., Liberati, David M., White, Michael T., Diglio, Clement A., Brown, William J.
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Translocation
Biological and medical sciences
Blood
Bronchoalveolar Lavage Fluid - immunology
Cells, Cultured
E coli
Escherichia coli
Experiments
Flow cytometry
General aspects
Humans
IgA
IgG
Immunoglobulin A, Secretory - metabolism
Immunoglobulin G - metabolism
Medical sciences
Mortality
Neutrophil activation
Neutrophil Activation - immunology
Pneumonia
Pneumonia - immunology
Pneumonia, Bacterial - immunology
Rodents
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Summary:An exaggerated inflammatory response to infections including nosocomial pneumonia may be detrimental to the host and contribute to morbidity and mortality. Both secretory immunoglobulin A (SIgA) and IgG contribute to the immune defense of respiratory surfaces. However, their relative ability to protect against invasive infections and the resultant host inflammatory response are unknown and were the basis for this study. Calu-3 cell (a respiratory epithelial cell line) monolayers were established in a Transwell system (Costar Corp., Cambridge, MA). Escherichia coli and either polyclonal SIgA or IgG were inoculated into the apical chamber and neutrophils (polymorphonuclear neutrophils) were added to the basal chamber. PMN cytotoxic potential was indexed by %CD11b expression, superoxide anion (O 2−) production, and % elastase release. Bacterial translocation into the basal chamber was quantitated after log transformation. Calu-3 monolayer integrity was indexed by permeability to dextran fluorescein isothiocyanate. The addition of E coli to Calu-3/polymorphonuclear cocultures led to increases in O 2− generation, elastase release, and CD11b expression. These effects were diminished by the addition of SIgA but not IgG. A similar effect was noted with Calu-3 barrier function. SIgA may function to protect against microbial invasion of respiratory surfaces and protect against tissue injury from an exaggerated inflammatory response.
ISSN:0002-9610
1879-1883
DOI:10.1016/j.amjsurg.2005.10.041