Effect of sternotomy and extracorporeal circulation on pulmonary neutrophil kinetics in pigs

Pulmonary margination of neutrophils may contribute to lung damage after extracorporeal circulation for cardiac surgery. We evaluated single-pass pulmonary neutrophil kinetics using the multiple indicator-dilution technique in control pigs (n = 10), after sternotomy alone (sterno, n = 10) or after 3...

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Bibliographic Details
Published in:Basic research in cardiology 2006-03, Vol.101 (2), p.133-139
Main Authors: Salamand, Agnés, Schwab, Andreas J, Merhi, Yahye, Perrault, Louis P, Simard, André, Dupuis, Jocelyn
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion - immunology
Extracorporeal Circulation - adverse effects
Lung - immunology
Neutrophil Infiltration - immunology
Neutrophils
Sternum - surgery
Swine
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Summary:Pulmonary margination of neutrophils may contribute to lung damage after extracorporeal circulation for cardiac surgery. We evaluated single-pass pulmonary neutrophil kinetics using the multiple indicator-dilution technique in control pigs (n = 10), after sternotomy alone (sterno, n = 10) or after 30 min of observation following a period of 90 min extracorporeal circulation (n = 7). Blood neutrophils increased in the control and sterno groups (p < 0.05) but remained unchanged in the extracorporeal circulation group. The transfer coefficient for neutrophil margination from the circulating to the lung-marginated pool (k(c-m)) and pulmonary neutrophil clearance (Cl(c-m)) were similar between the three groups. There was an inverse correlation between k(c-m) and the degree of lung tissue perfusion evaluated from the tracer-accessible extravascular lung water (r = -0.54, p < 0.01). There was no arterio-venous gradient of neutrophils in any of the groups, suggesting a dynamic equilibrium of the margination/demargination processes. We conclude that extracorporeal circulation does not significantly modify single pass pulmonary neutrophil kinetics 30 min after reperfusion. The rate of neutrophil margination to the tracer-accessible lung tissue suggests that lung tissue de-recruitment is associated with increased neutrophil margination.
ISSN:0300-8428
1435-1803
DOI:10.1007/s00395-005-0579-7