Enterocyte and M-Cell Transport of Native and Heat-Denatured Bovine β-Lactoglobulin:  Significance of Heat Denaturation

The three-dimensional structure, digestibility, and immunological properties of bovine β-lactoglobulin (β-lg) are modified by heat treatments used in processing of liquid milk products. Because it is not known if such treatments also modify the intestinal transport properties of β-lg, the transport...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-02, Vol.54 (4), p.1500-1507
Main Authors: Rytkönen, Jani, Valkonen, Kaija H, Virtanen, Vesa, Foxwell, Ruth A, Kyd, Jennelle M, Cripps, Allan W, Karttunen, Tuomo J
Format: Article
Language:English
Subjects:
allergens
Animals
Biological and medical sciences
Biological Transport
Caco-2 Cells
Cattle
Cell Line
cell lines
denaturation
enterocytes
Enterocytes - metabolism
Epithelial Cells - metabolism
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Handling, storage, packaging, transport
heat treatment
Hot Temperature
Humans
intestinal absorption
intestinal mucosa
lactoglobulins
Lactoglobulins - chemistry
Lactoglobulins - metabolism
M-cells
milk
Milk and cheese industries. Ice creams
protein conformation
Protein Denaturation
protein transport
Trypsin - metabolism
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Summary:The three-dimensional structure, digestibility, and immunological properties of bovine β-lactoglobulin (β-lg) are modified by heat treatments used in processing of liquid milk products. Because it is not known if such treatments also modify the intestinal transport properties of β-lg, the transport of native and heat-denatured bovine β-lg was investigated in experimental cell models using Caco-2 cells and M cells. Transport of β-lg labeled with a fluorescent marker was followed with fluorometric measurements, electrophoretic analyses, and fluorescence microscopy. The data show that both cell types transported native β-lg more efficiently than they did heat-denatured β-lg. In addition, M cells transported native β-lg more than Caco-2 cells. Transport of native and heat-denatured β-lg was transcellular. The electrophoretic data also suggest that heat-denatured β-lg may have degraded more than native β-lg during the transport. Keywords: β-Lactoglobulin; M cell; Caco-2 cell; heat denaturation; transport
ISSN:0021-8561
1520-5118
DOI:10.1021/jf052309d