Derivation of human embryonic stem cells in defined conditions

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that i...

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Bibliographic Details
Published in:Nature biotechnology 2006-02, Vol.24 (2), p.185-187
Main Authors: Thomson, James A, Ludwig, Tenneille E, Levenstein, Mark E, Jones, Jeffrey M, Berggren, W Travis, Mitchen, Erika R, Frane, Jennifer L, Crandall, Leann J, Daigh, Christine A, Conard, Kevin R, Piekarczyk, Marian S, Llanas, Rachel A
Format: Article
Language:English
Subjects:
Animal cells
Biological and medical sciences
Biotechnology
Cell Culture Techniques - methods
Cell Differentiation
Cell Line
Cell Proliferation
Cell Survival
Culture Media - chemistry
Culture Media - metabolism
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Genetic recombination
Humans
Methods. Procedures. Technologies
Proteins
Stem cells
Stem Cells - cytology
Stem Cells - physiology
Tissue Engineering - methods
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Summary:We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt1177