Evaluation of a novel kit for the rapid detection of extended-spectrum beta-lactamases

Since a method of rapidly detecting extended-spectrum beta-lactamase (ESBL) production in gram-negative isolates from patients with severe infection is urgently required, the present study of a novel commercial kit was conducted. The Cica-Beta Test I (Kanto Chemical, Tokyo, Japan) is designed for th...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2006, Vol.25 (1), p.49-51
Main Authors: COLODNER, R, REZNIK, B, GAL, V, YAMAZAKI, H, HANAKI, H, KUBO, R
Format: Article
Language:English
Subjects:
Bacteriological Techniques - methods
Bacteriological Techniques - standards
beta-Lactamases - analysis
beta-Lactamases - biosynthesis
Biological and medical sciences
Escherichia coli - classification
Escherichia coli - enzymology
Escherichia coli - isolation & purification
Humans
Infectious diseases
Klebsiella - classification
Klebsiella - enzymology
Klebsiella - isolation & purification
Medical sciences
Phenotype
Proteus mirabilis - classification
Proteus mirabilis - enzymology
Proteus mirabilis - isolation & purification
Reagent Kits, Diagnostic
Sensitivity and Specificity
Time Factors
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Summary:Since a method of rapidly detecting extended-spectrum beta-lactamase (ESBL) production in gram-negative isolates from patients with severe infection is urgently required, the present study of a novel commercial kit was conducted. The Cica-Beta Test I (Kanto Chemical, Tokyo, Japan) is designed for the rapid detection of ESBL in gram-negative bacteria directly from isolated colonies in a 15-min protocol. In this study, a total of 304 strains of Klebsiella spp., Escherichia coli and Proteus mirabilis were tested using the novel kit and the phenotypic confirmatory disk test using cefotaxime and ceftazidime with and without clavulanate. The kit showed 95.5 and 98.1% sensitivity and specificity, respectively, as compared to the disk test, and thus proved to be an appropriate tool for the rapid detection of ESBL.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-005-0074-y