DNA methylation status of the IL8 gene promoter in oral cells of smokers and non-smokers with chronic periodontitis

Aim: This study analysed the status of DNA methylation in the promoter region of the IL8 gene in oral mucosa cells from healthy, smoker and non‐smoker subjects with chronic periodontitis and compared these findings among groups with mRNA levels. Material and Methods: Genomic DNA from epithelial oral...

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Bibliographic Details
Published in:Journal of clinical periodontology 2009-09, Vol.36 (9), p.719-725
Main Authors: Oliveira, Naila F. P., Damm, Gilcy R., Andia, Denise C., Salmon, Cristiane, Nociti Jr, Francisco H., Line, Sérgio R. P., De Souza, Ana Paula
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Case-Control Studies
Chronic Periodontitis - genetics
Chronic Periodontitis - metabolism
CpG Islands
Dentistry
DNA Methylation
Epigenesis, Genetic
epigenetic
Epithelial Cells - metabolism
Facial bones, jaws, teeth, parodontium: diseases, semeiology
Female
Humans
IL-8
inflammation
Interleukin-8 - genetics
Male
Medical sciences
methylation
Middle Aged
Mouth Mucosa - cytology
Mouth Mucosa - metabolism
Non tumoral diseases
Otorhinolaryngology. Stomatology
periodontitis
Polymerase Chain Reaction
Promoter Regions, Genetic
Salmonidae
smokers
Smoking - genetics
Smoking - metabolism
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Summary:Aim: This study analysed the status of DNA methylation in the promoter region of the IL8 gene in oral mucosa cells from healthy, smoker and non‐smoker subjects with chronic periodontitis and compared these findings among groups with mRNA levels. Material and Methods: Genomic DNA from epithelial oral cells of 41 healthy subjects, 30 smokers with chronic periodontitis and 40 non‐smokers with chronic periodontitis were purified and modified by sodium bisulphite. Genomic DNA from blood leucocytes and gingival cells from biopsies of 13 subjects of each group were also purified and modified by sodium bisulphite. Modified DNA was submitted by methylation‐specific polymerase chain reaction (PCR) (MSP), electrophoresed on 10% polyacrylamide gels and stained with SYBR Gold. Total RNA from gingival cells was also isolated using the TRIzol reagent, and real‐time PCR performance was used to detect the levels of interleukin‐8 mRNA. Results: Our results indicate that individuals with chronic periodontitis, independent of smoking habit, have a higher percentage of hipomethylation of the IL8 gene than those controls in epithelial oral cells (p
ISSN:0303-6979
1600-051X
DOI:10.1111/j.1600-051X.2009.01446.x