Peptide-induced immune protection of CD8+ T cell-deficient mice against Friend retrovirus-induced disease

CD8+ CTLs and virus-neutralizing antibodies have been associated with spontaneous and vaccine-induced immune control of retroviral infections. We previously showed that a single immunization with an env gene-encoded CD4+ T cell epitope protected mice against fatal Friend retrovirus infection. Here,...

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Bibliographic Details
Published in:International immunology 2006-01, Vol.18 (1), p.183-198
Main Authors: Kawabata, Hiroyuki, Niwa, Atsuko, Tsuji-Kawahara, Sachiyo, Uenishi, Hirohide, Iwanami, Norimasa, Matsukuma, Hideaki, Abe, Hiroyuki, Tabata, Nobutada, Matsumura, Haruo, Miyazawa, Masaaki
Format: Article
Language:English
Subjects:
7-AAD    7-aminoactinomycin D
Animals
Antibodies, Viral - immunology
APC    antigen-presenting cell
B cell-deficient
B-Lymphocytes
B6    C57BL/6
Bone Marrow - immunology
Bone Marrow - virology
CB6F1    (BALB/c × C56BL/6)F1
CD4+ T
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes
ED50    dose required to induce 50% of the maximum response
epitope
Epitopes, T-Lymphocyte - administration & dosage
Epitopes, T-Lymphocyte - immunology
F-MuLV    Friend murine leukemia virus
FBS    fetal bovine serum
Friend murine leukemia virus - immunology
FV    Friend retrovirus complex
Gene Products, env - administration & dosage
Gene Products, env - immunology
Leukemia, Experimental - immunology
Leukemia, Experimental - prevention & control
Mice
Mice, Inbred BALB C
Mice, Knockout
PBBS    phosphate-buffered balanced salt solution
PID    post-infection day
Retroviridae Infections - immunology
Retroviridae Infections - prevention & control
SFFU    spleen focus-forming unit
SFFV    spleen focus-forming virus
Spleen - immunology
Spleen - virology
Tumor Virus Infections - immunology
Tumor Virus Infections - prevention & control
Vaccination - methods
vaccine
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
β2-microglobulin-deficient
β2m    β2-microglobulin
μMT    Ig μ-chain membrane exon-targeted
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Summary:CD8+ CTLs and virus-neutralizing antibodies have been associated with spontaneous and vaccine-induced immune control of retroviral infections. We previously showed that a single immunization with an env gene-encoded CD4+ T cell epitope protected mice against fatal Friend retrovirus infection. Here, we analyzed immune cell components required for the peptide-induced anti-retroviral protection. Mice lacking CD8+ T cells were nevertheless protected against Friend virus infection, while mice lacking B cells were not. Virus-producing cells both in the spleen and bone marrow decreased rapidly in their number and became undetectable by 4 weeks after infection in the majority of the peptide-immunized animals even in the absence of CD8+ T cells. In the vaccinated animals the production and class switching of virus-neutralizing and anti-leukemia cell antibodies were facilitated; however, virus-induced erythroid cell expansion was suppressed before neutralizing antibodies became detectable in the serum. Further, the numbers of virus-producing cells in the spleen and bone marrow in the early stage of the infection were smaller in the peptide-immunized than in unimmunized control mice in the absence of B cells. Thus, peptide immunization facilitates both early cellular and late humoral immune responses that lead to the effective control of the retrovirus-induced disease, but CD8+ T cells are not crucial for the elimination of virus-infected cells in the peptide-primed animals.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/dxh361