Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts

Within this study, we investigated whether the polyunsaturated fatty acids (PUFA)-rich nature of rumen protozoa is a consequence of ingestion of PUFA-rich chloroplasts. Four Hereford x Friesian steers were offered hay [low 18:3 (n-3) and low chlorophyll concentration] followed by freshly cut perenni...

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Bibliographic Details
Published in:FEMS microbiology ecology 2009-09, Vol.69 (3), p.461-471
Main Authors: Huws, Sharon A, Kim, Eun J, Kingston-Smith, Alison H, Lee, Michael R.F, Muetzel, Stefan M, Cookson, Alan R, Newbold, Charles J, Wallace, R. John, Scollan, Nigel D
Format: Article
Language:English
Subjects:
Animal Feed - analysis
Animal, plant and microbial ecology
Animals
Bacteria - genetics
Bacteria - metabolism
Biological and medical sciences
Cattle
Chlorophyll
Chlorophyll - metabolism
chloroplast
Chloroplasts
Chloroplasts - metabolism
Confocal microscopy
DGGE
DNA, Bacterial - genetics
DNA, Protozoan - genetics
Ecology
Eukaryota - genetics
Eukaryota - metabolism
fatty acid
Fatty acids
Fatty Acids, Unsaturated - metabolism
Fundamental and applied biological sciences. Psychology
Grasses
Ingestion
Linoleic acid
Male
Microbial ecology
Microbiology
Microscopy
planktonic and attached bacteria
Polyunsaturated fatty acids
Protozoa
QPCR
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 18S - genetics
Rumen
Rumen - microbiology
rumen protozoa
Transmission electron microscopy
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Summary:Within this study, we investigated whether the polyunsaturated fatty acids (PUFA)-rich nature of rumen protozoa is a consequence of ingestion of PUFA-rich chloroplasts. Four Hereford x Friesian steers were offered hay [low 18:3 (n-3) and low chlorophyll concentration] followed by freshly cut perennial ryegrass [high 18:3 (n-3) and high chlorophyll concentration] for 16 days. On the 14th and 16th days, rumen protozoa as well as attached and planktonic bacteria were fractionated 1 h before (-1 h), 2 and 6 h postfeeding, and their fatty acid concentrations determined. Protozoa fractionated from fresh grass-fed steers were richer (P
ISSN:0168-6496
1574-6941
DOI:10.1111/j.1574-6941.2009.00717.x