An evolutionary conserved role of Wnt signaling in stem cell fate decision

Wnt/Frizzled/ß-catenin-based signaling systems play diverse roles in metazoan development, being involved not only in the establishment of body axes in embryogenesis but also in regulating stem cell fate in mammalian post-embryonic development. We have studied the role the canonical Wnt cascade play...

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Bibliographic Details
Published in:Developmental biology 2006, Vol.289 (1), p.91-99
Main Authors: Teo, Regina, Möhrlen, Frank, Plickert, Günter, Müller, Werner A., Frank, Uri
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - pharmacology
Amino Acid Sequence
Animals
beta Catenin - analysis
beta Catenin - metabolism
Cell Differentiation - genetics
Cell Nucleus - chemistry
Cnidaria
Cytoplasm - chemistry
DNA, Complementary - genetics
Evolution, Molecular
Frizzled
Frizzled Receptors - genetics
Frizzled Receptors - physiology
Glycogen Synthase Kinase 3 - antagonists & inhibitors
Glycogen Synthase Kinase 3 - metabolism
Hydractinia
Hydrozoa - cytology
Molecular Sequence Data
Nematocytes
Nerve cells
RNA, Messenger - analysis
RNA, Messenger - metabolism
Signal Transduction
Stem cells
Stem Cells - chemistry
Stem Cells - cytology
Transcription, Genetic
Wnt
Wnt Proteins - physiology
ß-catenin
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Summary:Wnt/Frizzled/ß-catenin-based signaling systems play diverse roles in metazoan development, being involved not only in the establishment of body axes in embryogenesis but also in regulating stem cell fate in mammalian post-embryonic development. We have studied the role the canonical Wnt cascade plays in stem cell fate determination in Hydractinia, a member of the ancient metazoan phylum Cnidaria, by analyzing two key molecules in this pathway, frizzled and ß-catenin, and blocking GSK-3. Generally, frizzled was expressed in cells able to divide but absent in post-mitotic, terminally differentiated cells such as nerve cells and nematocytes. Transcripts of frizzled were identified in all embryonic stages beginning with maternal transcripts in the oocyte. Following gastrulation and in the planula larva, frizzled expression concentrated in the central endodermal mass from which the first interstitial stem cells and their derivatives arise. In post-metamorphic development, high levels of frizzled transcripts were detected in interstitial stem cells. Activating downstream events of the Wnt-cascade in the post-metamorphic life phase by blocking GSK-3 with paullones induced recruitment of nematocytes and nerve cells from the pool of interstitial stem cells. Terminal differentiation was preceded by an initial burst of proliferation of frizzled-positive i-cells. In activated i-cells, ß-catenin appeared in the cytoplasm, later in the nucleus. It was subsequently again observed in the cytoplasm and eventually faded out during terminal differentiation. Our results suggest an ancient role of Wnt signaling in stem cell fate determination.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2005.10.009