Estimation of the effective orientation of the SHG source in primary cortical neurons

In this paper we provide, for the first time to our knowledge, the effective orientation of the SHG source in cultured cortical neuronal processes in vitro. This is done by the use of the polarization sensitive second harmonic generation (PSHG) imaging microscopy technique. By performing a pixel-lev...

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Bibliographic Details
Published in:Optics express 2009-08, Vol.17 (16), p.14418-14425
Main Authors: Psilodimitrakopoulos, Sotiris, Petegnief, Valerie, Soria, Guadalupe, Amat-Roldan, Ivan, Artigas, David, Planas, Anna M, Loza-Alvarez, Pablo
Format: Article
Language:English
Subjects:
Animals
Cerebral Cortex - cytology
Computer Simulation
Computer-Aided Design
Equipment Design
Equipment Failure Analysis
Image Enhancement - instrumentation
Image Enhancement - methods
Light
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Models, Theoretical
Neurons - cytology
Optical Devices
Rats
Rats, Sprague-Dawley
Scattering, Radiation
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Summary:In this paper we provide, for the first time to our knowledge, the effective orientation of the SHG source in cultured cortical neuronal processes in vitro. This is done by the use of the polarization sensitive second harmonic generation (PSHG) imaging microscopy technique. By performing a pixel-level resolution analysis we found that the SHG dipole source has a distribution of angles centered at thetae =33.96 degrees , with a bandwidth of Deltathetae = 12.85 degrees . This orientation can be related with the molecular geometry of the tubulin heterodimmer contained in microtubules.
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.17.014418