Poly I:C induces an antiviral state against Ictalurid Herpesvirus 1 and Mx1 transcription in the channel catfish ( Ictalurus punctatus)

In vivo studies were carried out to investigate the protective effect of the interferon inducer poly I:C against channel catfish virus (CCV). Channel catfish were stimulated by intraperitoneal injection of 50 μg of poly I:C or PBS at various days prior to immersion challenge with CCV. Mortality in t...

Full description

Saved in:
Bibliographic Details
Published in:Developmental and comparative immunology 2005, Vol.29 (7), p.627-635
Main Authors: Plant, Karen P., Harbottle, Heather, Thune, Ronald L.
Format: Article
Language:English
Subjects:
Animals
Antiviral
Channel catfish virus
GTP-Binding Proteins - genetics
GTP-Binding Proteins - immunology
GTP-Binding Proteins - metabolism
Herpesviridae - immunology
Herpesviridae Infections - immunology
Herpesviridae Infections - mortality
Ictaluridae - immunology
Interferon Inducers - pharmacology
Myxovirus Resistance Proteins
Organ Specificity
Poly I-C - pharmacology
Poly I:C
RNA, Messenger - metabolism
Transcription, Genetic - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In vivo studies were carried out to investigate the protective effect of the interferon inducer poly I:C against channel catfish virus (CCV). Channel catfish were stimulated by intraperitoneal injection of 50 μg of poly I:C or PBS at various days prior to immersion challenge with CCV. Mortality in the poly I:C group was significantly reduced from 70% to 3% at day 1 compared to the PBS controls. Mortality increased at day 3 but was still significantly different from the PBS controls. Mx1 transcription was significantly higher only at day 1. In an additional study Mx1 transcription was monitored in the liver, kidney, gills, spleen, and intestine at various time points post-stimulation with either poly I:C or CCV. Mx1 mRNA was significantly elevated in all organs only at day 1 post-injection with poly I:C. In response to CCV, Mx1 transcription was not significantly elevated until day 3 post-challenge, but remained elevated in certain organs until day 7.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2004.09.008