Activation-dependent phases of T cells distinguished by use of optical tweezers and near infrared Raman spectroscopy

Near-infrared Raman spectroscopy may provide a highly sensitive, noninvasive means to identify activation status of leukocytes. The purpose of the current study was to establish Raman spectroscopic characteristics of T cell activation. Activation of the RsL.11 T cell clone in vitro with Con A result...

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Bibliographic Details
Published in:Journal of immunological methods 2005-02, Vol.297 (1), p.53-60
Main Authors: Mannie, Mark D., McConnell, Thomas J., Xie, Changan, Li, Yong-qing
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blastogenesis
Cell Line
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Lymphocyte Activation
Molecular immunology
Optical tweezers
Raman spectroscopy
Rats
Rats, Inbred Lew
Spectroscopy, Near-Infrared - methods
Spectrum Analysis, Raman - methods
T cell activation
T-Lymphocytes - immunology
Techniques
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Summary:Near-infrared Raman spectroscopy may provide a highly sensitive, noninvasive means to identify activation status of leukocytes. The purpose of the current study was to establish Raman spectroscopic characteristics of T cell activation. Activation of the RsL.11 T cell clone in vitro with Con A resulted in specific decrements in band intensities at 785, 1048, 1093, and 1376 cm −1 but did not alter a majority of other band intensities including those at 1004 cm −1 (phenylalanine) and 1660 cm −1 (amide bonds). Activation-dependent decrements in these band intensities occurred subsequent to IL-2 production and correlated closely with T cell blastogenesis. Activation-dependent decrements in these band intensities were not strictly a function of cell size because the same observations were noted in size-controlled comparisons of resting and activated T cells. Like the RsL.11 clone, freshly isolated thymocytes that were activated by Con A or IL-2 showed decrements in particular emissions. These findings indicate that near-infrared Raman spectroscopy can be used as a noninvasive technique to reveal the activation status of single living T cells.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2004.11.020