Histological Basis of the Porcine Femoral Artery for Vascular Research

Summary The similarities between the porcine and human cardiovascular systems make the pig a useful animal model for the study of vascular biology. However, a standardized method is needed to describe the normal histological properties of porcine arteries in order to evaluate pathologic lesions in f...

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Bibliographic Details
Published in:Anatomia, histologia, embryologia histologia, embryologia, 2005-04, Vol.34 (2), p.105-111
Main Authors: Solanes, N., Rigol, M., Ramírez, J., Segalés, J., Roqué, M., Marimon, J.M., Pérez-Villa, F., Roig, E., Sanz, G., Heras, M.
Format: Article
Language:English
Subjects:
Animals
Disease Models, Animal
Endothelium, Vascular - anatomy & histology
Endothelium, Vascular - cytology
Female
Femoral Artery - anatomy & histology
Femoral Artery - cytology
Histocytochemistry
Humans
Immunohistochemistry
Lymph Nodes - anatomy & histology
Lymph Nodes - cytology
Male
Muscle, Smooth, Vascular - anatomy & histology
Muscle, Smooth, Vascular - cytology
Reference Values
Swine - anatomy & histology
Thigh - blood supply
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Summary:Summary The similarities between the porcine and human cardiovascular systems make the pig a useful animal model for the study of vascular biology. However, a standardized method is needed to describe the normal histological properties of porcine arteries in order to evaluate pathologic lesions in future studies. Descriptive and morphometric analyses were done on 16 porcine femoral arteries. For these purposes, three histological stains (haematoxylin eosin, Masson's trichrome, and orcein), four immunohistochemical methods (using antibodies anti‐α‐actin, anti‐CD3, anti‐L1 and anti‐lysozyme), and a glycohistochemical method (using Dolichos biflorus lectin) were performed. The porcine femoral arteries evaluated had a mean total area of 6.25 ± 1.99 mm2 and a diameter of 2.79 ± 0.41 mm. The majority of the total area was occupied by the medial layer (42.97 ± 5.38%) and was mainly constituted by smooth muscle cells (94.58 ± 2.65%). All the cell markers used reacted with porcine paraffin‐embedded tissue. However, the anti‐lysozyme antibody was excluded from this histological analysis because of cytoplasmatic reactivity in smooth muscle cells. In summary, this study proposes histological methods to describe the normal characteristics of the porcine femoral artery and raises the possibility of applying this methodology in future studies on porcine vascular research.
ISSN:0340-2096
1439-0264
DOI:10.1111/j.1439-0264.2004.00580.x