Combinatorial microarray analysis revealing Arabidopsis genes implicated in cytokinin responses through the His-Asp phosphorelay circuitry

In Arabidopsis thaliana, the immediate early response of plants to cytokinin is formulated as the multistep histidine kinase (AHK)-histidine-containing phosphotransmitter (AHP)-response regulator (ARR) phosphorelay signaling circuitry, which is initiated by the cytokinin receptor histidine protein k...

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Bibliographic Details
Published in:Plant and cell physiology 2005-02, Vol.46 (2), p.339-355
Main Authors: Kiba, T.(Nagoya Univ. (Japan). Faculty of Agriculture), Naitou, T, Koizumi, N, Yamashino, T, Sakakibara, H, Mizuno, T
Format: Article
Language:English
Subjects:
AHK
AHP
Arabidopsis - genetics
Arabidopsis histidine kinase
Arabidopsis histidine-containing phosphotransmitter
Arabidopsis response regulator
ARABIDOPSIS THALIANA
ARR
aspartic acid
Aspartic Acid - metabolism
basic helix–loop–helix
bHLH
Cells, Cultured
CITOQUININAS
Cytokinin
CYTOKININE
CYTOKININS
Cytokinins - metabolism
Cytokinins - pharmacology
Databases, Genetic
dimethylsulfoxide
DMSO
EXPRESION GENICA
EXPRESSION DES GENES
fold change
GENE
GENE EXPRESSION
gene expression regulation
Gene Expression Regulation, Plant - drug effects
Gene Expression Regulation, Plant - physiology
GENES
gibberellin
histidine
Histidine - metabolism
methyl jasmonate
Microarray
microarray technology
MJA
molecular sequence data
nucleotide sequences
Oligonucleotide Array Sequence Analysis - methods
Phosphorelay
Phosphorylation
Plant Growth Regulators - pharmacology
plant proteins
plant response
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
reverse transcription–polymerase chain reaction
RT–PCR
Signal transduction
Signal Transduction - genetics
transcription factors
TRANSGENIC PLANTS
Up-Regulation - genetics
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Summary:In Arabidopsis thaliana, the immediate early response of plants to cytokinin is formulated as the multistep histidine kinase (AHK)-histidine-containing phosphotransmitter (AHP)-response regulator (ARR) phosphorelay signaling circuitry, which is initiated by the cytokinin receptor histidine protein kinases. In the hope of finding components (or genes) that function downstream of the cytokinin-mediated His-Asp phosphorelay signaling circuitry, we carried out genome-wide microarray analyses. To this end, we used a combinatorial microarray strategy by employing not only wild-type plants, but also certain transgenic lines in which the cytokinin-mediated His-Asp phosphorelay signaling circuitry has been genetically manipulated. These transgenic lines employed were ARR21-0verexpressing and ARR22-0verexpressing plants, each of which exhibits a characteristic phenotype with regard to the cytokinin-mediated His-Asp phosphorelay The results of extensive microarray analyses with these plants allowed us systematically to identify a certain number of genes that were up-regulated at the level of transcription in response to cytokinin directly or indirectly. Among them, some representatives were examined further in wild-type plants to support the idea that certain genes encoding transcription factors are rapidly and specifically induced at the level of transcription by cytokinin in a manner similar to that of the type-A ARR genes, which are the hallmarks of the His-Asp phosphorelay signaling circuitry. Several interesting transcription factors were thus identified as being cytokinin responsive, including those belonging to the AP2/EREBP family, MYB family, GATA family or bHLH family. Including these, the presented list of cytokinin-up-regulated genes (214) will provide us with valuable bases for understanding the His-Asp phosphorelay in A. thaliana.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pci033