Effect of post-excitation radius on ion abundance, mass measurement accuracy, and isotopic distributions in Fourier transform ion cyclotron resonance mass spectrometry

We report an evaluation of a modern Fourier transform ion cyclotron resonance mass spectrometry (FT‐ICR‐MS) instrument to determine the general trend of post‐excitation radius on total ion abundance, mass measurement accuracy, and isotopic distributions for internally calibrated mass spectra. The op...

Full description

Saved in:
Bibliographic Details
Published in:Rapid communications in mass spectrometry 2005-01, Vol.19 (7), p.915-918
Main Authors: Hawkridge, Adam M., Nepomuceno, Angelito I., Lovik, Stephanie L., Mason, Christopher J., Muddiman, David C.
Format: Article
Language:English
Subjects:
Bradykinin - analysis
Ions
Isotopes
Reproducibility of Results
Spectroscopy, Fourier Transform Infrared - instrumentation
Spectroscopy, Fourier Transform Infrared - methods
Tritium
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report an evaluation of a modern Fourier transform ion cyclotron resonance mass spectrometry (FT‐ICR‐MS) instrument to determine the general trend of post‐excitation radius on total ion abundance, mass measurement accuracy, and isotopic distributions for internally calibrated mass spectra. The optimum post‐excitation radius was determined using total ion abundance, mass measurement accuracy (MMA), and isotope ratios. However, despite the utility of internal calibration for achieving ultimate MMA, the internal calibrant ions were insufficient for compensating for sub‐optimum ICR cell conditions. The findings presented herein underscore the importance of determining the optimal post‐excitation radius in FT‐ICR‐MS to achieve high ion abundance (low limits of detection), high MMA, and valid isotopic distributions. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.1871