Human hemokinin-1 and human hemokinin-1(4–11), mammalian tachykinin peptides, suppress proliferation and induce differentiation in HL-60 cells

Human hemokinin-1 (h HK-1) and its truncated form h HK-1(4–11) are mammalian tachykinin peptides encoded by the TAC4 gene identified in human, and the biological functions of these peptides have not been well investigated. The tachykinins have shown immuno-regulatory activities in humans. In the pre...

Full description

Saved in:
Bibliographic Details
Published in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2009-08, Vol.30 (8), p.1514-1522
Main Authors: Zhao, You-Li, Tao, Yan, Fu, Cai-Yun, Kong, Zi-Qing, Chen, Qiang, Wang, Rui
Format: Article
Language:English
Subjects:
Benzamides - pharmacology
Biological and medical sciences
Cell Cycle - drug effects
Cell Differentiation - drug effects
Cell Proliferation - drug effects
Differentiation
Fundamental and applied biological sciences. Psychology
h HK-1 and h HK-1(4–11)
HL-60
HL-60 Cells
Humans
Neurokinin-1 Receptor Antagonists
Neurotransmitter Agents - pharmacology
Piperidines - pharmacology
Proliferation inhibition
Quinuclidines - pharmacology
Receptors, Neurokinin-2 - antagonists & inhibitors
SR140333 and SR48968
Tachykinins - pharmacology
Vertebrates: endocrinology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human hemokinin-1 (h HK-1) and its truncated form h HK-1(4–11) are mammalian tachykinin peptides encoded by the TAC4 gene identified in human, and the biological functions of these peptides have not been well investigated. The tachykinins have shown immuno-regulatory activities in humans. In the present study, we investigated the effects of h HK-1 and h HK-1(4–11) on the proliferation and differentiation of a human promyelocyte leukemia cell line, HL-60. It is noteworthy that h HK-1 (1–300 μM) displayed inhibitory effects on the proliferation of HL-60 cells in a dose- and time-dependent manner. The effect of suppressing proliferation induced by these peptides was accompanied by an accumulation of cell cycle in the S phase. Moreover, this peptide induced differentiation of HL-60 cells by significantly increasing the NBT-reduction activity. The effects induced by h HK-1(4–11) on HL-60 cells were similar to that of h HK-1, indicating that it is the active fragment of h HK-1. However these effects induced by h HK-1 or h HK-1(4–11) were not antagonized by the NK 1 receptor antagonist SR140333 or the NK 2 receptor antagonist SR48968. All the results indicated that h HK-1 and h HK-1(4–11) were able to significantly inhibit proliferation and induce differentiation and S phase arrest of a human promyelocyte leukemia cell line HL-60, which may not be mediated through the activation of classical tachykinin NK 1 receptors and tachykinin NK 2 receptors. Our observations also implied that h HK-1 and h HK-1(4–11) could act as immunomodulatory factors in cancer chemotherapy.
ISSN:0196-9781
1873-5169
DOI:10.1016/j.peptides.2009.04.024