Expression of cytokeratin 17 mRNA in oral squamous cell carcinoma cells obtained by brush biopsy: preliminary results

Background:  The aim of this study was to determine the detection of cytokeratin (CK) mRNA in oral squamous cell carcinoma (OSCC) cells and to evaluate the CK relevance for OSCC diagnosis in a brush biopsy test. Methods:  Fifty‐two pairs of OSCC cells and normal oral mucosal cells were obtained by b...

Full description

Saved in:
Bibliographic Details
Published in:Journal of oral pathology & medicine 2009-07, Vol.38 (6), p.530-534
Main Authors: Toyoshima, T., Koch, F., Kaemmerer, P., Vairaktaris, E., Al-Nawas, B., Wagner, W.
Format: Article
Language:English
Subjects:
Biomarkers, Tumor - analysis
brush biopsy
Carcinoma, Squamous Cell - diagnosis
Carcinoma, Squamous Cell - pathology
Carcinoma, Squamous Cell - secondary
Cell Line, Tumor
Cytodiagnosis - instrumentation
Cytodiagnosis - methods
cytokeratin
Dentistry
diagnostic marker
Gene Expression Regulation
Humans
Keratin-17 - analysis
Keratin-19 - analysis
Keratin-20 - analysis
Lymphatic Metastasis - pathology
Mouth Mucosa - pathology
Mouth Neoplasms - diagnosis
Mouth Neoplasms - pathology
Neoplasm Staging
oral squamous cell carcinoma
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
SYBR green real-time quantitative reverse transcriptase polymerase chain reaction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background:  The aim of this study was to determine the detection of cytokeratin (CK) mRNA in oral squamous cell carcinoma (OSCC) cells and to evaluate the CK relevance for OSCC diagnosis in a brush biopsy test. Methods:  Fifty‐two pairs of OSCC cells and normal oral mucosal cells were obtained by brush biopsy from OSCC patients. mRNA was extracted from cell pellets for real‐time quantitative reverse transcriptase polymerase chain reaction (RT‐qPCR). The over‐expression levels of CK 17, CK 19 and CK 20 mRNA in OSCC cells were examined by SYBR green real‐time RT‐qPCR. Results:  Compared to normal mucosal cells, the over‐expression of CK 17 mRNA was detectable in 40 OSCC cells (76.9%), that of CK 19 mRNA in 19 (36.5%), while that of CK 20 mRNA was not detectable. Compared with CK 19, the mean value of CK 17 mRNA expression level was significantly higher in all 52 patients (P 
ISSN:0904-2512
1600-0714
DOI:10.1111/j.1600-0714.2009.00748.x