Viability and adipogenic potential of human adipose tissue processed cell population obtained from pump-assisted and syringe-assisted liposuction

Correcting soft tissue defects by autologous fat grafting has been a routine procedure in plastic surgery. Liposuction material for autologous fat grafting is normally obtained using a hand-held syringe. The pump-assisted liposuction technique is not used because of the belief that cells in the harv...

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Bibliographic Details
Published in:Journal of dermatological science 2005-03, Vol.37 (3), p.169-176
Main Authors: Leong, David T.W., Hutmacher, Dietmar W., Chew, F.T., Lim, Thiam-Chye
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - pathology
Adipogenic
Adipose Tissue - metabolism
Adipose Tissue - pathology
Autologous lipoinjection
Azo Compounds - pharmacology
Cell Differentiation
Cell Lineage
Cell Proliferation
Cell Survival
Coloring Agents - pharmacology
Culture Media - pharmacology
Fat grafting
Humans
Lipectomy - instrumentation
Lipectomy - methods
Lipid Metabolism
Mesoderm - metabolism
Oxazines - pharmacology
Pump-assisted liposuction
Suction
Syringes
Time Factors
Xanthenes - pharmacology
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Summary:Correcting soft tissue defects by autologous fat grafting has been a routine procedure in plastic surgery. Liposuction material for autologous fat grafting is normally obtained using a hand-held syringe. The pump-assisted liposuction technique is not used because of the belief that cells in the harvested fat tissue are necrotic from the physical forces created by the vacuum pump liposuction machine. The metabolism and adipogenic potential of cultured mesenchymal precursor cells processed from pump and syringe lipoaspirates were studied. Metabolic estimates were determined with alamarBlue ™ assay. These cells were also induced along the adipogenic lineage with adipogenic induction factors. The extent of adipogenic differentiation was measured using a digital photo counting software. The cells obtained by pump-assisted liposuction are viable, have comparable metabolic activity and adipogenic potential which are comparable to cells using the syringe for aspiration. The implications of this study are that pump-assisted liposuction might be a possible alternative to aspirating adipose tissue for reimplantation during reconstruction procedures.
ISSN:0923-1811
1873-569X
DOI:10.1016/j.jdermsci.2004.11.009