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The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum
Recently, we described the molecular identification of dendritic cell‐specific TrAnsMembrane protein (DC‐STAMP), a multimembrane‐spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC‐STAMP (mDC...
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| Published in: | Journal of leukocyte biology 2005-03, Vol.77 (3), p.337-343 |
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| Main Authors: | , , , , , , , , , , |
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| container_end_page | 343 |
| container_issue | 3 |
| container_start_page | 337 |
| container_title | Journal of leukocyte biology |
| container_volume | 77 |
| creator | Eleveld‐Trancikova, Dagmar Triantis, Vassilis Moulin, Veronique Looman, Maaike W. G. Wijers, Mietske Fransen, Jack A. M. Lemckert, Angelique A. C. Havenga, Menzo J. E. Figdor, Carl G. Janssen, Richard A. J. Adema, Gosse J. |
| description | Recently, we described the molecular identification of dendritic cell‐specific TrAnsMembrane protein (DC‐STAMP), a multimembrane‐spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC‐STAMP (mDC‐STAMP) as well as the characterization of the DC‐STAMP protein. The results demonstrate that mDC‐STAMP is over 90% homologous to hDC‐STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC‐STAMP expression is enhanced by interleukin‐4 and down‐regulated upon DC maturation. Analysis of differently tagged DC‐STAMP proteins further demonstrates that hDC‐STAMP and mDC‐STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC‐STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC‐STAMP‐green fluorescent protein fusion protein. These data imply that DC‐STAMP may exert its effect in the ER. |
| doi_str_mv | 10.1189/jlb.0804441 |
| format | article |
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G. ; Wijers, Mietske ; Fransen, Jack A. M. ; Lemckert, Angelique A. C. ; Havenga, Menzo J. E. ; Figdor, Carl G. ; Janssen, Richard A. J. ; Adema, Gosse J.</creator><creatorcontrib>Eleveld‐Trancikova, Dagmar ; Triantis, Vassilis ; Moulin, Veronique ; Looman, Maaike W. G. ; Wijers, Mietske ; Fransen, Jack A. M. ; Lemckert, Angelique A. C. ; Havenga, Menzo J. E. ; Figdor, Carl G. ; Janssen, Richard A. J. ; Adema, Gosse J.</creatorcontrib><description>Recently, we described the molecular identification of dendritic cell‐specific TrAnsMembrane protein (DC‐STAMP), a multimembrane‐spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC‐STAMP (mDC‐STAMP) as well as the characterization of the DC‐STAMP protein. The results demonstrate that mDC‐STAMP is over 90% homologous to hDC‐STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC‐STAMP expression is enhanced by interleukin‐4 and down‐regulated upon DC maturation. Analysis of differently tagged DC‐STAMP proteins further demonstrates that hDC‐STAMP and mDC‐STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC‐STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC‐STAMP‐green fluorescent protein fusion protein. These data imply that DC‐STAMP may exert its effect in the ER.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1189/jlb.0804441</identifier><identifier>PMID: 15601667</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>Adenovirus ; Amino Acid Sequence ; Animals ; APC ; Cell Line ; Cloning, Molecular ; CLSM ; Dendritic Cells - metabolism ; Endoplasmic Reticulum - metabolism ; Gene Expression Profiling ; Green Fluorescent Proteins - genetics ; human ; Humans ; immunobiology ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; mouse ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Rats ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid</subject><ispartof>Journal of leukocyte biology, 2005-03, Vol.77 (3), p.337-343</ispartof><rights>2005 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4927-358075d3d535bae140d374da505f95e3d5c77b48e60ac45fa51b80fdc979c1b53</citedby><cites>FETCH-LOGICAL-c4927-358075d3d535bae140d374da505f95e3d5c77b48e60ac45fa51b80fdc979c1b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1189%2Fjlb.0804441$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1189%2Fjlb.0804441$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15601667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eleveld‐Trancikova, Dagmar</creatorcontrib><creatorcontrib>Triantis, Vassilis</creatorcontrib><creatorcontrib>Moulin, Veronique</creatorcontrib><creatorcontrib>Looman, Maaike W. G.</creatorcontrib><creatorcontrib>Wijers, Mietske</creatorcontrib><creatorcontrib>Fransen, Jack A. M.</creatorcontrib><creatorcontrib>Lemckert, Angelique A. C.</creatorcontrib><creatorcontrib>Havenga, Menzo J. E.</creatorcontrib><creatorcontrib>Figdor, Carl G.</creatorcontrib><creatorcontrib>Janssen, Richard A. J.</creatorcontrib><creatorcontrib>Adema, Gosse J.</creatorcontrib><title>The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>Recently, we described the molecular identification of dendritic cell‐specific TrAnsMembrane protein (DC‐STAMP), a multimembrane‐spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC‐STAMP (mDC‐STAMP) as well as the characterization of the DC‐STAMP protein. The results demonstrate that mDC‐STAMP is over 90% homologous to hDC‐STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC‐STAMP expression is enhanced by interleukin‐4 and down‐regulated upon DC maturation. Analysis of differently tagged DC‐STAMP proteins further demonstrates that hDC‐STAMP and mDC‐STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC‐STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC‐STAMP‐green fluorescent protein fusion protein. These data imply that DC‐STAMP may exert its effect in the ER.</description><subject>Adenovirus</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>APC</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>CLSM</subject><subject>Dendritic Cells - metabolism</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>human</subject><subject>Humans</subject><subject>immunobiology</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Sequence Data</subject><subject>mouse</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Rats</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkU2P0zAQhi0EYrsLJ-7IF7igLOPajpPjUlg-VAQS5Ww59oS6cpJiJ0Tl1-OolbjBydLomWc88xLyjMEtY1X9-hCaW6hACMEekBWreVXwUvGHZAVKsEIKgCtyndIBAPi6hMfkiskSWFmqFTns9kgd9i760VtqMYTCYfS_0NFjHEb0PX27Kb7t7j5_pT7Rvf-xDydqhz5hXCDTOxoGa4L_jYmOAx2zMPuGYzCpy8qIWTyFqXtCHrUmJHx6eW_I9_t3u82HYvvl_cfN3bawol6rgssKlHTcSS4bg0yA40o4I0G2tcRct0o1osISjBWyNZI1FbTO1qq2rJH8hrw8e_P_f06YRt35tCxmehympEslyoqB-i_IlOTriosMvjqDNg4pRWz1MfrOxJNmoJcMdM5AXzLI9POLdmo6dH_Zy9EzAGdg9gFP_3LpT9s3wPnS8uLcstx_9hF16kwIecJaz_OslOZ64f4AZc6d_A</recordid><startdate>200503</startdate><enddate>200503</enddate><creator>Eleveld‐Trancikova, Dagmar</creator><creator>Triantis, Vassilis</creator><creator>Moulin, Veronique</creator><creator>Looman, Maaike W. G.</creator><creator>Wijers, Mietske</creator><creator>Fransen, Jack A. M.</creator><creator>Lemckert, Angelique A. C.</creator><creator>Havenga, Menzo J. E.</creator><creator>Figdor, Carl G.</creator><creator>Janssen, Richard A. J.</creator><creator>Adema, Gosse J.</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200503</creationdate><title>The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum</title><author>Eleveld‐Trancikova, Dagmar ; Triantis, Vassilis ; Moulin, Veronique ; Looman, Maaike W. G. ; Wijers, Mietske ; Fransen, Jack A. M. ; Lemckert, Angelique A. C. ; Havenga, Menzo J. E. ; Figdor, Carl G. ; Janssen, Richard A. J. ; Adema, Gosse J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4927-358075d3d535bae140d374da505f95e3d5c77b48e60ac45fa51b80fdc979c1b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenovirus</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>APC</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>CLSM</topic><topic>Dendritic Cells - metabolism</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>human</topic><topic>Humans</topic><topic>immunobiology</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Sequence Data</topic><topic>mouse</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Rats</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eleveld‐Trancikova, Dagmar</creatorcontrib><creatorcontrib>Triantis, Vassilis</creatorcontrib><creatorcontrib>Moulin, Veronique</creatorcontrib><creatorcontrib>Looman, Maaike W. 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J.</au><au>Adema, Gosse J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2005-03</date><risdate>2005</risdate><volume>77</volume><issue>3</issue><spage>337</spage><epage>343</epage><pages>337-343</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><abstract>Recently, we described the molecular identification of dendritic cell‐specific TrAnsMembrane protein (DC‐STAMP), a multimembrane‐spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC‐STAMP (mDC‐STAMP) as well as the characterization of the DC‐STAMP protein. The results demonstrate that mDC‐STAMP is over 90% homologous to hDC‐STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC‐STAMP expression is enhanced by interleukin‐4 and down‐regulated upon DC maturation. Analysis of differently tagged DC‐STAMP proteins further demonstrates that hDC‐STAMP and mDC‐STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC‐STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC‐STAMP‐green fluorescent protein fusion protein. These data imply that DC‐STAMP may exert its effect in the ER.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>15601667</pmid><doi>10.1189/jlb.0804441</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of leukocyte biology, 2005-03, Vol.77 (3), p.337-343 |
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| language | eng |
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| source | Wiley Online Library; Oxford Academic Journals (OUP) |
| subjects | Adenovirus Amino Acid Sequence Animals APC Cell Line Cloning, Molecular CLSM Dendritic Cells - metabolism Endoplasmic Reticulum - metabolism Gene Expression Profiling Green Fluorescent Proteins - genetics human Humans immunobiology Membrane Proteins - genetics Membrane Proteins - metabolism Mice Mice, Inbred C57BL Molecular Sequence Data mouse Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Rats RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid |
| title | The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum |
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