The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum

Recently, we described the molecular identification of dendritic cell‐specific TrAnsMembrane protein (DC‐STAMP), a multimembrane‐spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC‐STAMP (mDC...

Full description

Saved in:
Bibliographic Details
Published in:Journal of leukocyte biology 2005-03, Vol.77 (3), p.337-343
Main Authors: Eleveld‐Trancikova, Dagmar, Triantis, Vassilis, Moulin, Veronique, Looman, Maaike W. G., Wijers, Mietske, Fransen, Jack A. M., Lemckert, Angelique A. C., Havenga, Menzo J. E., Figdor, Carl G., Janssen, Richard A. J., Adema, Gosse J.
Format: Article
Language:English
Subjects:
Adenovirus
Amino Acid Sequence
Animals
APC
Cell Line
Cloning, Molecular
CLSM
Dendritic Cells - metabolism
Endoplasmic Reticulum - metabolism
Gene Expression Profiling
Green Fluorescent Proteins - genetics
human
Humans
immunobiology
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mice
Mice, Inbred C57BL
Molecular Sequence Data
mouse
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recently, we described the molecular identification of dendritic cell‐specific TrAnsMembrane protein (DC‐STAMP), a multimembrane‐spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC‐STAMP (mDC‐STAMP) as well as the characterization of the DC‐STAMP protein. The results demonstrate that mDC‐STAMP is over 90% homologous to hDC‐STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC‐STAMP expression is enhanced by interleukin‐4 and down‐regulated upon DC maturation. Analysis of differently tagged DC‐STAMP proteins further demonstrates that hDC‐STAMP and mDC‐STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC‐STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC‐STAMP‐green fluorescent protein fusion protein. These data imply that DC‐STAMP may exert its effect in the ER.
ISSN:0741-5400
1938-3673
DOI:10.1189/jlb.0804441