Development and organization of the central vacuole of Acetabularia acetabulum

• Here we analyzed the shape of the central vacuole of Acetabularia acetabulum by visualizing its development during diplophase (from juvenility through reproduction) and haplophase (from meiosis through mating). • Light microscopy and whole-organism applications of a pH-sensitive dye, neutral red,...

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Published in:	The New phytologist 2005-03, Vol.165 (3), p.731-746
Main Authors:	Ngo, Duc A., Garland, Phillip A., Mandoli, Dina F.
Format:	Article
Language:	eng
Subjects:	Acetabularia Acetabularia - cytology Acetabularia - physiology Acetabularia - ultrastructure Acetabularia acetabulum Algae algae and seaweeds Biological and medical sciences Cell physiology Cells, cell elements: structure and function central vacuole Coloring Agents Cytoplasm development Developmental biology dye movement Dyes Fundamental and applied biological sciences. Psychology morphogenesis Neutral Red organelle Pelvic bones Plant cells plant development Plant physiology and development Sea water Surface areas Thallus Vacuoles Vacuoles - physiology
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Summary:	• Here we analyzed the shape of the central vacuole of Acetabularia acetabulum by visualizing its development during diplophase (from juvenility through reproduction) and haplophase (from meiosis through mating). • Light microscopy and whole-organism applications of a pH-sensitive dye, neutral red, were used to visualize the anatomy of the central vacuole. We studied connectivity within the thallus by locally applying dye to morphologically distinct regions (rhizoid, stalk, apex, hairs) and observing dye movements. • In vegetative thalli most of the rhizoid, stalk and young hairs stained with dye. In reproductive structures (caps, gametangia) dye also stained the majority of the interiors. When applied to small areas, dye moved at different rates through each region of the thallus (e.g. within the stalk). Dye moved from younger hairs, but not from older hairs, into the stalk. Errors in incorporation of central vacuole into gametangia occurred at $
ISSN:	0028-646X 1469-8137