Liposomes as carriers for dermal delivery of tretinoin: in vitro evaluation of drug permeation and vesicle–skin interaction

The influence of liposome composition, size, lamellarity and charge on the (trans)dermal delivery of tretinoin (TRA) was studied. For this purpose we studied both multilamellar (MLV) or unilamellar (UV) liposomes. Positively or negatively charged liposomes were obtained using either hydrogenated (Ph...

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Bibliographic Details
Published in:Journal of controlled release 2005-03, Vol.103 (1), p.123-136
Main Authors: Sinico, Chiara, Manconi, Maria, Peppi, Marcello, Lai, Francesco, Valenti, Donatella, Fadda, Anna Maria
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Dermis - drug effects
Dermis - metabolism
Drug Carriers - administration & dosage
Drug Carriers - pharmacokinetics
Drug Evaluation, Preclinical - methods
General pharmacology
In Vitro Techniques
Liposomes
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Pig skin
Skin Absorption - drug effects
Skin Absorption - physiology
Swine
Topical application
Transmission electron microscopy
Tretinoin
Tretinoin - administration & dosage
Tretinoin - pharmacokinetics
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Summary:The influence of liposome composition, size, lamellarity and charge on the (trans)dermal delivery of tretinoin (TRA) was studied. For this purpose we studied both multilamellar (MLV) or unilamellar (UV) liposomes. Positively or negatively charged liposomes were obtained using either hydrogenated (Phospholipon®90H) or non-hydrogenated soy phosphatidylcholine (Phospholipon®90) and cholesterol, in combination with stearylamine or dicetylphosphate. Liposomal formulations were characterized by transmission electron microscopy (TEM) and optical and light polarized microscopy for vesicle formation and morphology, and by dynamic laser light scattering for size distribution. In order to obtain more information about the stability and the thermodynamic activity of the liposomal tretinoin, TRA diffusion through a lipophilic membrane was investigated. The effect of the vesicular incorporation of tretinoin on its accumulation into the newborn pig skin was also studied. The experiments were performed in vitro using Franz cells in occlusive conditions and were compared to three different controls. The tretinoin amount delivered through and accumulated in the several skin layers was detected by HPLC. Furthermore, TEM in combination with osmium tetroxide was used to visualize the skin structure after the liposomal administration. Overall obtained results showed that liposomes may be an interesting carrier for tretinoin in skin disease treatment, when appropriate formulations are used. In particular, negatively charged liposomes strongly improved newborn pig skin hydration and TRA retention, though no evidence of intact vesicle penetration was found.
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2004.11.020