Placental insulin and insulin-like growth factor I receptors in normal and preeclamptic pregnancies

To assess the ligand binding characteristics of insulin and insulin-like growth factor I receptors in normal and preeclamptic placentas. This study was done cross sectionally in trophoblast membranes obtained from 10 normotensive and 9 preeclamptic pregnant women. The affinity and number of binding...

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Bibliographic Details
Published in:Clinical biochemistry 2005-03, Vol.38 (3), p.243-247
Main Authors: Díaz, Eulises, Cárdenas, Mario, Ariza, Ana C., Larrea, Fernando, Halhali, Ali
Format: Article
Language:English
Subjects:
Adult
Birth Weight
Blood Pressure
Case-Control Studies
Cross-Sectional Studies
Female
Fetal Development
Humans
IGF-1 receptor
Infant, Newborn
Insulin receptor
Placenta
Placenta - metabolism
Pre-Eclampsia - metabolism
Pre-Eclampsia - physiopathology
Preeclampsia
Pregnancy
Receptor, IGF Type 1 - metabolism
Receptor, Insulin - metabolism
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Summary:To assess the ligand binding characteristics of insulin and insulin-like growth factor I receptors in normal and preeclamptic placentas. This study was done cross sectionally in trophoblast membranes obtained from 10 normotensive and 9 preeclamptic pregnant women. The affinity and number of binding sites of insulin and insulin-like growth factor I receptors were assessed by binding assays and Scatchard plot analysis. Placental and newborn birth weights were significantly ( P < 0.001) lower in the preeclamptic group. The K d values of placental insulin receptors (IR) were significantly higher in the preeclamptic group than in the normotensive group (1.08 ± 0.24 × 10 −9 vs. 0.81 ± 0.13 × 10 −9 M, P < 0.01), without differences in the number of receptors. In contrast, no differences were observed in the affinity and the number of insulin-like growth factor I receptors (IGF-1R) between groups. Placental weight was associated negatively with the K d values of IR ( P < 0.05) and positively with the number of placental IGF-1R ( P < 0.05); while newborn birth weight was associated positively with the number of IGF-1R ( P < 0.05). In addition, both systolic and diastolic blood pressure correlated significantly with K d values of placental IR ( P < 0.01). These data demonstrate that preeclampsia is associated with low placental IR affinity. In addition, this study suggests an association between the affinity of IR and number of IGF-1R with placental and/or fetal growth. Furthermore, high blood pressure may affect the affinity of placental IR, but not the affinity or number of placental IGF-1 receptors.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2004.10.013