candidate for Lr19, an exotic gene conditioning leaf rust resistance in wheat

Lr19, one of the few widely effective genes conferring resistance to leaf rust in wheat, was transferred from the wild relative Thinopyrum ponticum to durum wheat. Since Lr19 confers a hypersensitive response to the pathogen, it was considered likely that the gene would be a member of the major nucl...

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Bibliographic Details
Published in:Functional & integrative genomics 2009-08, Vol.9 (3), p.325-334
Main Authors: Gennaro, Andrea, Koebner, Robert M. D, Ceoloni, Carla
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal Genetics and Genomics
Base Sequence
Basidiomycota - pathogenicity
Biochemistry
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Cell Biology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
General aspects
Genes, Plant
Genetic markers
Genomics
Immunity, Innate - genetics
Life Sciences
Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects)
Microbial Genetics and Genomics
Molecular Sequence Data
Original Paper
Plant biology
Plant Diseases - microbiology
Plant Genetics and Genomics
Plant Proteins - genetics
Plant Proteins - metabolism
Plant resistance
Recombination, Genetic
Sequence Analysis, DNA
Thinopyrum ponticum
Triticum - genetics
Triticum - microbiology
Triticum aestivum
Wheat
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Summary:Lr19, one of the few widely effective genes conferring resistance to leaf rust in wheat, was transferred from the wild relative Thinopyrum ponticum to durum wheat. Since Lr19 confers a hypersensitive response to the pathogen, it was considered likely that the gene would be a member of the major nucleotide-binding site (NBS)-leucine-rich repeat (LRR) plant R gene family. NBS profiling, based on PCR amplification of conserved NBS motifs, was applied to durum wheat-Th. ponticum recombinant lines involving different segments of the alien 7AgL chromosome arm, carrying or lacking Lr19. Differential PCR products were isolated and sequenced. From one such sequence (AG15), tightly linked to Lr19, a 4,121-bp full-length cDNA was obtained. Its deduced 1,258 amino acid sequence has the characteristic NBS-LRR domains of plant R gene products and includes a coiled-coil (CC) region typical of monocots. The genomic DNA sequence showed the presence of two exons and a short intron upstream of the predicted stop codon. Homology searches revealed considerable identity of AG15 with the cloned wheat resistance gene Pm3a and a lower similarity with wheat Lr1, Lr21, and Lr10. Quantitative PCR on leaf-rust-infected and non-infected Lr19 carriers proved AG15 to be constitutively expressed, as is common for R genes.
ISSN:1438-793X
1438-7948
DOI:10.1007/s10142-009-0115-1