red beet (Beta vulgaris) UDP-glucosyltransferase gene induced by wounding, bacterial infiltration and oxidative stress

Mechanical wounding, infiltration with P. syringae or A. tumefaciens, and exposure to an H2O2-generating system (Glc/Glc oxidase) induce betacyanin synthesis in red beet (Beta vulgaris) leaves. These conditions also induced the expression of BvGT, a gene encoding a glucosyltransferase (GT) from Beta...

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Bibliographic Details
Published in:Journal of experimental botany 2005-02, Vol.56 (412), p.605-611
Main Authors: Sepúlveda-Jiménez, Gabriela, Rueda-Benítez, Patricia, Porta, Helena, Rocha-Sosa, Mario
Format: Article
Language:English
Subjects:
1,3-beta-glucan synthase
Agrobacterium tumefaciens
Amino Acid Sequence
amino acid sequences
antisense DNA
bacterial diseases of plants
Bacterial infiltration
Beets
Beta vulgaris
Beta vulgaris - drug effects
Beta vulgaris - enzymology
Beta vulgaris - microbiology
betacyanin
Betacyanins
Biological and medical sciences
Biphenyl Compounds - pharmacology
Enzyme Induction
enzyme inhibitors
Enzymes
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - physiology
genes
Genes. Genome
glucosyltransferase
Glucosyltransferases - biosynthesis
Glucosyltransferases - genetics
hexosyltransferases
Leaves
mechanical damage
Messenger RNA
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Molecular Structure
NADPH Oxidases - antagonists & inhibitors
NADPH Oxidases - metabolism
nucleotide sequences
Onium Compounds - pharmacology
Oxidases
Oxidative Stress
oxidoreductases
Pigments, Biological - biosynthesis
Pigments, Biological - chemistry
Plant Diseases - microbiology
Plant Leaves
plant pathogenic bacteria
Plants
Polymerase chain reaction
Pseudomonas syringae
Reactive oxygen species
red beet
RESEARCH PAPER
RNA
Sequence Alignment
Sequence Homology, Amino Acid
transgenic plants
UDP-glucosyltransferase
wounding
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Summary:Mechanical wounding, infiltration with P. syringae or A. tumefaciens, and exposure to an H2O2-generating system (Glc/Glc oxidase) induce betacyanin synthesis in red beet (Beta vulgaris) leaves. These conditions also induced the expression of BvGT, a gene encoding a glucosyltransferase (GT) from Beta vulgaris. BvGT has a high similarity to Dorotheanthus bellidiformis betanidin-5 GT involved in betacyanin synthesis. Furthermore, the transient expression of a BvGT antisense construct resulted in the reduction of BvGT transcript accumulation and betanin synthesis, suggesting a role for this gene product in betacyanin glucosylation. In addition, the NADPH oxidase inhibitor, diphenylene iodonium (DPI), inhibited the accumulation of the BvGT transcript in response to infiltration with Agrobacterium tumefaciens. Hence, this result suggests that ROS produced by a plasma membrane NADPH oxidase may act as a signal to induce BvGT expression, necessary for betanin synthesis after wounding and bacterial infiltration.
ISSN:0022-0957
1460-2431
1460-2431
DOI:10.1093/jxb/eri036