Prevalence of human herpesvirus-8 infection in HIV-positive patients with and without Kaposi’s sarcoma in Hungary

Human herpesvirus-8 (HHV-8) infection of 130 Hungarian HIV-positive individuals with or without Kaposi’s sarcoma was investigated from 158 serum and 122 peripheral blood samples using anti-latency-associated nuclear antigen (LANA) indirect immunofluorescence assay (IFA), recombinant orf65 and orfK8....

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Published in:FEMS immunology and medical microbiology 2005-02, Vol.43 (2), p.265-268
Main Authors: Szalai, Erika, Gerlei, Zsuzsanna, Szlávik, János, Szládek, Györgyi, Patel, Ravi, Hunyadi, János, Gergely, Lajos, Juhász, Attila
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Antibodies, Viral - blood
Antigens
Antigens, Viral - immunology
Assaying
Biological and medical sciences
Bisexual
Bisexuality
Blood
Blood donors
Blotting, Western
Child
Deoxyribonucleic acid
DNA
Enzyme-Linked Immunosorbent Assay
Epidemiology
Female
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Genes, Viral
Herpesviridae Infections - complications
Herpesviridae Infections - epidemiology
Herpesvirus 8, Human - immunology
Herpesvirus 8, Human - isolation & purification
HIV
HIV Infections - complications
Human herpesvirus 8
Human immunodeficiency virus
Human viral diseases
Humans
Hungary - epidemiology
Immunoassays
Immunofluorescence
Infections
Infectious diseases
Kaposi's sarcoma
Kaposis sarcoma
Latency
Latency-associated nuclear antigen
Male
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Nuclear Proteins - immunology
orf65
orf73
orfK8.1
Peripheral blood
Polymerase Chain Reaction
Prevalence
Recombinant Proteins
Risk Factors
Sarcoma
Sarcoma, Kaposi - epidemiology
Serology
Sexual Behavior
Tumors
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Proteins - immunology
Virology
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Summary:Human herpesvirus-8 (HHV-8) infection of 130 Hungarian HIV-positive individuals with or without Kaposi’s sarcoma was investigated from 158 serum and 122 peripheral blood samples using anti-latency-associated nuclear antigen (LANA) indirect immunofluorescence assay (IFA), recombinant orf65 and orfK8.1 antigen enzyme-linked immunosorbent assays (ELISAs), Western blot assays and orf26 specific nested polymerase chain reaction (PCR). The overall prevalence of HHV-8 infection was found to be 31.5% (41/130) among the Hungarian HIV-positive patients. This seroprevalence rate is 7–11-fold higher than that of healthy HIV-negative blood donors in Hungary. The highest prevalence of HHV-8 infection (36.1%, 35/97) was observed in homo- or bisexual patients. Similar to the serologic results, HHV-8 DNA was not always detectable in all serial samples previously shown to be positive for HHV-8 DNA.
ISSN:0928-8244
1574-695X
2049-632X
DOI:10.1016/j.femsim.2004.08.012