Opto-electronic DNA chip: high performance chip reading with an all-electric interface

Reading of DNA chips is usually based on fluorescence labeling of hybridised target molecules. Combined with the use of confocal fluorescence scanners, this approach shows very high performances in terms of accuracy and sensitivity. However, fluorescence readers remain costly and cumbersome. This pr...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2005-03, Vol.20 (9), p.1813-1820
Main Authors: Mallard, Frédéric, Marchand, Gilles, Ginot, Frédéric, Campagnolo, Raymond
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Chemiluminescence
DNA - analysis
DNA - chemistry
DNA - genetics
DNA chip
Electrochemistry - instrumentation
Electrochemistry - methods
Electronics
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
HRP
Luminescent Measurements - instrumentation
Luminescent Measurements - methods
Methods. Procedures. Technologies
Microchemistry - instrumentation
Microchemistry - methods
Oligonucleotide Array Sequence Analysis - instrumentation
Oligonucleotide Array Sequence Analysis - methods
Optics and Photonics - instrumentation
Photodetector array
Signal Processing, Computer-Assisted - instrumentation
Various methods and equipments
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Summary:Reading of DNA chips is usually based on fluorescence labeling of hybridised target molecules. Combined with the use of confocal fluorescence scanners, this approach shows very high performances in terms of accuracy and sensitivity. However, fluorescence readers remain costly and cumbersome. This prevents the use of DNA chips as a decentralised testing tool. Electrical monitoring of hybridisation is one way to reduce the cost and size of the reader. However, the multiplexing of electric detection-based systems in a miniaturised form remains challenging. Here, we present a system based on the use of a low cost CMOS photodetector array as a solid support for a DNA chip, coupled with revelation by enzyme-catalysed chemiluminescence. This system is shown to allow the detection of low pM target concentrations with a 3 logs dynamic range on dense DNA microarrays, with excellent inter-spot reproducibility. Combining electric interface and high analytical performances, this opto-electronic DNA chip is one attractive solution for nucleic acids detection and analysis in disposable, fully automatised, total analysis systems developed for decentralised testing.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2004.07.031