Variations in bovine milk oligosaccharides during early and middle lactation stages analyzed by high-performance liquid chromatography-chip/mass spectrometry

Milk oligosaccharides (OS) are not only a source of nutrition for newborns, but also provide numerous important biological functions including the prevention of pathogen binding to the intestinal epithelium and serving as nutritive sources for beneficial bacteria. High-performance mass spectrometry...

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Bibliographic Details
Published in:Journal of dairy science 2009-07, Vol.92 (7), p.2991-3001
Main Authors: Tao, N., DePeters, E.J., German, J.B., Grimm, R., Lebrilla, C.B.
Format: Article
Language:English
Subjects:
Animal productions
Animals
anionic oligosaccharides
anions
Biological and medical sciences
bovine milk
breed differences
carbohydrate composition
Cattle - physiology
chemical structure
Chromatography, High Pressure Liquid
Colostrum - chemistry
early lactation
Female
Food industries
fucosylated oligosaccharides
Fundamental and applied biological sciences. Psychology
Ions - chemistry
Lactation - physiology
lactation stage
Mass Spectrometry
middle lactation
milk
Milk - chemistry
milk analysis
Milk and cheese industries. Ice creams
milk composition
N-acetylneuraminic acid
N-glycolylneuraminic acid
neuraminic acid
oligosaccharide
oligosaccharides
Oligosaccharides - chemistry
Species Specificity
Terrestrial animal productions
Time Factors
Vertebrates
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Summary:Milk oligosaccharides (OS) are not only a source of nutrition for newborns, but also provide numerous important biological functions including the prevention of pathogen binding to the intestinal epithelium and serving as nutritive sources for beneficial bacteria. High-performance mass spectrometry and separation methods were used to evaluate changes of bovine milk oligosaccharides (bMO) in different lactation stages. Previously, 40 bMO were identified in bovine milk with many consisting of short oligomeric chains that were less complex than human milk oligosaccharides (hMO). The bMO are also significantly more anionic than hMO, with nearly 70% in measured abundances containing either N-acetylneuraminic acid or N-glycolylneuraminic acid, and no fucosylated OS. In this study, we examined factors that could affect the abundances of bMO including stage of lactation and breed. The total concentrations dropped rapidly in the first several days of lactation. Moreover, the anionic oligosaccharides (including N-glycolylneuraminic acid) decreased more rapidly compared with the neutral oligosaccharides.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2008-1642