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Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes
The neonatal Fc receptor (FcRn) for IgG has been shown to be responsible for IgG transport and to be involved in IgG catabolism. In this study, we show expression of FcRn in normal human epidermal keratinocytes. By RT-PCR, we demonstrate the FcRn α-chain mRNA obtained from cultured keratinocytes cre...
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Published in: | Journal of investigative dermatology 2005-01, Vol.124 (1), p.132-139 |
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creator | Cauza, Karla Hinterhuber, Gabriele Dingelmaier-Hovorka, Ruth Brugger, Karin Klosner, Gabriele Horvat, Reinhard Wolff, Klaus Foedinger, Dagmar |
description | The neonatal Fc receptor (FcRn) for IgG has been shown to be responsible for IgG transport and to be involved in IgG catabolism. In this study, we show expression of FcRn in normal human epidermal keratinocytes. By RT-PCR, we demonstrate the FcRn α-chain mRNA obtained from cultured keratinocytes creating a 457 bp product as confirmed by sequence analysis. Northern blot analysis shows a 1.5 kb transcript. Real-time PCR reveals consistent expression of FcRn α-chain mRNA in human keratinocytes from different donors. Anti-FcRn α2-extracellular domain and anti-FcRn cytoplasmic tail antibody (Ab) directed against defined antigenic targets were generated and used for immunoblotting and immunoprecipitation revealing protein expression of the 46 kDa FcRn α-chain. By immunofluorescence microscopy, we find granular–vesicular staining for FcRn α-chain in keratinocytes. Fluorescence-activated cell sorting analysis gives predominantly an intracellular distribution of FcRn in keratinocytes. Biochemically, we demonstrate Fc-dependent binding of human IgG at acidic pH. In normal human epidermis, we find a cytoplasmic vesicular staining of predominantly basal and suprabasal keratinocytes. In summary, we demonstrate expression of a functional FcRn in normal human epidermal keratinocytes. These findings further emphasize the role of keratinocytes as immunomodulating cells in inflammatory and immunologic processes of the skin. |
doi_str_mv | 10.1111/j.0022-202X.2004.23542.x |
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In this study, we show expression of FcRn in normal human epidermal keratinocytes. By RT-PCR, we demonstrate the FcRn α-chain mRNA obtained from cultured keratinocytes creating a 457 bp product as confirmed by sequence analysis. Northern blot analysis shows a 1.5 kb transcript. Real-time PCR reveals consistent expression of FcRn α-chain mRNA in human keratinocytes from different donors. Anti-FcRn α2-extracellular domain and anti-FcRn cytoplasmic tail antibody (Ab) directed against defined antigenic targets were generated and used for immunoblotting and immunoprecipitation revealing protein expression of the 46 kDa FcRn α-chain. By immunofluorescence microscopy, we find granular–vesicular staining for FcRn α-chain in keratinocytes. Fluorescence-activated cell sorting analysis gives predominantly an intracellular distribution of FcRn in keratinocytes. Biochemically, we demonstrate Fc-dependent binding of human IgG at acidic pH. In normal human epidermis, we find a cytoplasmic vesicular staining of predominantly basal and suprabasal keratinocytes. In summary, we demonstrate expression of a functional FcRn in normal human epidermal keratinocytes. These findings further emphasize the role of keratinocytes as immunomodulating cells in inflammatory and immunologic processes of the skin.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/j.0022-202X.2004.23542.x</identifier><identifier>PMID: 15654966</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Adult ; Base Sequence ; Biological and medical sciences ; Cells, Cultured ; Dermatology ; epidermis ; Epidermis - cytology ; Epidermis - physiology ; Fc receptors ; Gene Expression - immunology ; Histocompatibility Antigens Class I ; Humans ; Hydrogen-Ion Concentration ; immunity ; Immunoglobulin G - immunology ; Immunoglobulin G - metabolism ; Keratinocytes - cytology ; Keratinocytes - physiology ; Medical sciences ; Molecular Sequence Data ; Protein Binding - immunology ; Receptors, Fc - genetics ; Receptors, Fc - immunology ; RNA, Messenger - analysis</subject><ispartof>Journal of investigative dermatology, 2005-01, Vol.124 (1), p.132-139</ispartof><rights>2005 The Society for Investigative Dermatology, Inc</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jan 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c598t-3aa32fdcbcb2e57f787a48d1fd22b11cab4d7d1a1a3d6dbbaab8f905780e459a3</citedby><cites>FETCH-LOGICAL-c598t-3aa32fdcbcb2e57f787a48d1fd22b11cab4d7d1a1a3d6dbbaab8f905780e459a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,4043,27956,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16589837$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15654966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cauza, Karla</creatorcontrib><creatorcontrib>Hinterhuber, Gabriele</creatorcontrib><creatorcontrib>Dingelmaier-Hovorka, Ruth</creatorcontrib><creatorcontrib>Brugger, Karin</creatorcontrib><creatorcontrib>Klosner, Gabriele</creatorcontrib><creatorcontrib>Horvat, Reinhard</creatorcontrib><creatorcontrib>Wolff, Klaus</creatorcontrib><creatorcontrib>Foedinger, Dagmar</creatorcontrib><title>Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>The neonatal Fc receptor (FcRn) for IgG has been shown to be responsible for IgG transport and to be involved in IgG catabolism. In this study, we show expression of FcRn in normal human epidermal keratinocytes. By RT-PCR, we demonstrate the FcRn α-chain mRNA obtained from cultured keratinocytes creating a 457 bp product as confirmed by sequence analysis. Northern blot analysis shows a 1.5 kb transcript. Real-time PCR reveals consistent expression of FcRn α-chain mRNA in human keratinocytes from different donors. Anti-FcRn α2-extracellular domain and anti-FcRn cytoplasmic tail antibody (Ab) directed against defined antigenic targets were generated and used for immunoblotting and immunoprecipitation revealing protein expression of the 46 kDa FcRn α-chain. By immunofluorescence microscopy, we find granular–vesicular staining for FcRn α-chain in keratinocytes. Fluorescence-activated cell sorting analysis gives predominantly an intracellular distribution of FcRn in keratinocytes. Biochemically, we demonstrate Fc-dependent binding of human IgG at acidic pH. In normal human epidermis, we find a cytoplasmic vesicular staining of predominantly basal and suprabasal keratinocytes. In summary, we demonstrate expression of a functional FcRn in normal human epidermal keratinocytes. These findings further emphasize the role of keratinocytes as immunomodulating cells in inflammatory and immunologic processes of the skin.</description><subject>Adult</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Dermatology</subject><subject>epidermis</subject><subject>Epidermis - cytology</subject><subject>Epidermis - physiology</subject><subject>Fc receptors</subject><subject>Gene Expression - immunology</subject><subject>Histocompatibility Antigens Class I</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>immunity</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin G - metabolism</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - physiology</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Protein Binding - immunology</subject><subject>Receptors, Fc - genetics</subject><subject>Receptors, Fc - immunology</subject><subject>RNA, Messenger - analysis</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkV9rFDEUxYModq1-BCUI-tQZ82cyyTzq0nYXK8JSwbeQSe5oltnMNpmR7bc34y4t-NIL4T7c3z3cnIMQpqSkuT5tS0IYKxhhP0tGSFUyLipWHp6hBRWMF1RW8jlaPEBn6FVKW0JoXQn1Ep1RUYuqqesFur087COk5IeAhw5f2U24wONvwN9WS7zsTUp4XWygNyM4vAEL-3GIuMtv_ev6AvuAV9POBPwVohl9GOz9COk1etGZPsGbUz9HP64ub5er4ub79Xr5-aawolFjwY3hrHO2tS0DITuppKmUo51jrKXUmrZy0lFDDXe1a1tjWtU1REhFoBKN4efo41F3H4e7CdKodz5Z6HsTYJiSriXPbNM8CTIiGy5kncH3_4HbYYohf0IzSriiVMxq6gjZOKQUodP76Hcm3mtK9JyP3urZej1br-d89L989CGvvjvpT-0O3OPiKZAMfDgBJlnTd9EE69MjVwvVKC4z9_bIBTNOER4AUROWpfL8y3EO2f8_HqJO1kOw4HwEO2o3-Kev_QspF7bk</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Cauza, Karla</creator><creator>Hinterhuber, Gabriele</creator><creator>Dingelmaier-Hovorka, Ruth</creator><creator>Brugger, Karin</creator><creator>Klosner, Gabriele</creator><creator>Horvat, Reinhard</creator><creator>Wolff, Klaus</creator><creator>Foedinger, Dagmar</creator><general>Elsevier Inc</general><general>Nature Publishing</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes</title><author>Cauza, Karla ; 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In this study, we show expression of FcRn in normal human epidermal keratinocytes. By RT-PCR, we demonstrate the FcRn α-chain mRNA obtained from cultured keratinocytes creating a 457 bp product as confirmed by sequence analysis. Northern blot analysis shows a 1.5 kb transcript. Real-time PCR reveals consistent expression of FcRn α-chain mRNA in human keratinocytes from different donors. Anti-FcRn α2-extracellular domain and anti-FcRn cytoplasmic tail antibody (Ab) directed against defined antigenic targets were generated and used for immunoblotting and immunoprecipitation revealing protein expression of the 46 kDa FcRn α-chain. By immunofluorescence microscopy, we find granular–vesicular staining for FcRn α-chain in keratinocytes. Fluorescence-activated cell sorting analysis gives predominantly an intracellular distribution of FcRn in keratinocytes. Biochemically, we demonstrate Fc-dependent binding of human IgG at acidic pH. In normal human epidermis, we find a cytoplasmic vesicular staining of predominantly basal and suprabasal keratinocytes. In summary, we demonstrate expression of a functional FcRn in normal human epidermal keratinocytes. These findings further emphasize the role of keratinocytes as immunomodulating cells in inflammatory and immunologic processes of the skin.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>15654966</pmid><doi>10.1111/j.0022-202X.2004.23542.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Base Sequence Biological and medical sciences Cells, Cultured Dermatology epidermis Epidermis - cytology Epidermis - physiology Fc receptors Gene Expression - immunology Histocompatibility Antigens Class I Humans Hydrogen-Ion Concentration immunity Immunoglobulin G - immunology Immunoglobulin G - metabolism Keratinocytes - cytology Keratinocytes - physiology Medical sciences Molecular Sequence Data Protein Binding - immunology Receptors, Fc - genetics Receptors, Fc - immunology RNA, Messenger - analysis |
title | Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes |
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