Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes

The neonatal Fc receptor (FcRn) for IgG has been shown to be responsible for IgG transport and to be involved in IgG catabolism. In this study, we show expression of FcRn in normal human epidermal keratinocytes. By RT-PCR, we demonstrate the FcRn α-chain mRNA obtained from cultured keratinocytes cre...

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Bibliographic Details
Published in:Journal of investigative dermatology 2005-01, Vol.124 (1), p.132-139
Main Authors: Cauza, Karla, Hinterhuber, Gabriele, Dingelmaier-Hovorka, Ruth, Brugger, Karin, Klosner, Gabriele, Horvat, Reinhard, Wolff, Klaus, Foedinger, Dagmar
Format: Article
Language:English
Subjects:
Adult
Base Sequence
Biological and medical sciences
Cells, Cultured
Dermatology
epidermis
Epidermis - cytology
Epidermis - physiology
Fc receptors
Gene Expression - immunology
Histocompatibility Antigens Class I
Humans
Hydrogen-Ion Concentration
immunity
Immunoglobulin G - immunology
Immunoglobulin G - metabolism
Keratinocytes - cytology
Keratinocytes - physiology
Medical sciences
Molecular Sequence Data
Protein Binding - immunology
Receptors, Fc - genetics
Receptors, Fc - immunology
RNA, Messenger - analysis
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Summary:The neonatal Fc receptor (FcRn) for IgG has been shown to be responsible for IgG transport and to be involved in IgG catabolism. In this study, we show expression of FcRn in normal human epidermal keratinocytes. By RT-PCR, we demonstrate the FcRn α-chain mRNA obtained from cultured keratinocytes creating a 457 bp product as confirmed by sequence analysis. Northern blot analysis shows a 1.5 kb transcript. Real-time PCR reveals consistent expression of FcRn α-chain mRNA in human keratinocytes from different donors. Anti-FcRn α2-extracellular domain and anti-FcRn cytoplasmic tail antibody (Ab) directed against defined antigenic targets were generated and used for immunoblotting and immunoprecipitation revealing protein expression of the 46 kDa FcRn α-chain. By immunofluorescence microscopy, we find granular–vesicular staining for FcRn α-chain in keratinocytes. Fluorescence-activated cell sorting analysis gives predominantly an intracellular distribution of FcRn in keratinocytes. Biochemically, we demonstrate Fc-dependent binding of human IgG at acidic pH. In normal human epidermis, we find a cytoplasmic vesicular staining of predominantly basal and suprabasal keratinocytes. In summary, we demonstrate expression of a functional FcRn in normal human epidermal keratinocytes. These findings further emphasize the role of keratinocytes as immunomodulating cells in inflammatory and immunologic processes of the skin.
ISSN:0022-202X
1523-1747
DOI:10.1111/j.0022-202X.2004.23542.x