Conversion of cholic acid and chenodeoxycholic acid into their 7-oxo derivatives by Bacteroides intestinalis AM-1 isolated from human feces

Secondary bile acid-producing bacteria were isolated from human feces to improve our appreciation of the functional diversity and redundancy of the intestinal microbiota. In total, 619 bacterial colonies were isolated using a nutrient-poor agar medium and the level of secondary bile acid formation w...

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Bibliographic Details
Published in:FEMS microbiology letters 2009-04, Vol.293 (2), p.263-270
Main Authors: Fukiya, Satoru, Arata, Miki, Kawashima, Hiroko, Yoshida, Daisuke, Kaneko, Maki, Minamida, Kimiko, Watanabe, Jun, Ogura, Yoshio, Uchida, Kiyohisa, Itoh, Kikuji, Wada, Masaru, Ito, Susumu, Yokota, Atsushi
Format: Article
Language:English
Subjects:
7-oxo-deoxycholic acid
7-oxo-lithocholic acid
7α-Hydroxysteroid dehydrogenase
Acids
Adult
Bacterial Typing Techniques
Bacteriology
Bacteroides - classification
Bacteroides - growth & development
Bacteroides - isolation & purification
Bacteroides - metabolism
Bacteroides fragilis
Bacteroides fragilis - metabolism
Bacteroides intestinalis
Bile
bile acid conversion
Biological and medical sciences
Biotransformation
Chenodeoxycholic acid
Chenodeoxycholic Acid - metabolism
Cholic acid
Cholic Acid - metabolism
Chromatography, Thin Layer
Culture
Culture Media - chemistry
Deoxycholic acid
Deoxycholic Acid - analogs & derivatives
Deoxycholic Acid - metabolism
Derivatives
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
E coli
Escherichia coli
Escherichia coli - metabolism
Feces
Feces - microbiology
Female
Fundamental and applied biological sciences. Psychology
Gene sequencing
Humans
intestinal bacteria
Intestinal microflora
intestinal microorganisms
Intestine
Liquid culture
Lithocholic Acid - analogs & derivatives
Lithocholic Acid - metabolism
Male
Metabolism. Enzymes
Microbiology
Microbiota
Phylogeny
Redundancy
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence Analysis, DNA
Strains (organisms)
Thin layer chromatography
Young Adult
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Summary:Secondary bile acid-producing bacteria were isolated from human feces to improve our appreciation of the functional diversity and redundancy of the intestinal microbiota. In total, 619 bacterial colonies were isolated using a nutrient-poor agar medium and the level of secondary bile acid formation was examined in each by a liquid culture, followed by thin-layer chromatography. Of five strains analyzed by 16S rRNA gene sequencing and biochemical testing, one was identified as Bacteroides intestinalis AM-1, which was not previously recognized as a secondary bile-acid producer. GC-MS revealed that B. intestinalis AM-1 converts cholic acid (CA) and chenodeoxycholic acid into their 7-oxo derivatives, 7-oxo-deoxycholic acid (7-oxo-DCA) and 7-oxo-lithocholic acid, respectively. Thus, B. intestinalis AM-1 possesses 7α-hydroxysteroid dehydrogenase (7α-HSDH) activity. In liquid culture, B. intestinalis AM-1 showed a relatively higher productivity of 7-oxo-DCA than Escherichia coli HB101 and Bacteroides fragilis JCM11019T, which are known to possess 7α-HSDH activity. The level of 7α-HSDH activity was higher in B. intestinalis AM-1 than in the other two strains under the conditions tested. The 7α-HSDH activity in each of the three strains is not induced by CA; instead, it is regulated in a growth phase-dependent manner.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2009.01531.x