Optimization of a transplant model to assess skin reconstitution from stem cell-enriched primary human keratinocyte populations

:  Given that an important functional attribute of stem cells in vivo is their ability to sustain tissue regeneration, we set out to establish a simple and easy technique to assess this property from candidate populations of human keratinocyte stem cells in an in vivo setting. Keratinocytes were ino...

Full description

Saved in:
Bibliographic Details
Published in:Experimental dermatology 2005-01, Vol.14 (1), p.60-69
Main Authors: Pouliot, Normand, Redvers, Richard P., Ellis, Sarah, Saunders, Nicholas A., Kaur, Pritinder
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Culture Techniques - methods
Cell Differentiation
Cell Movement
Cell Transplantation - methods
Coculture Techniques - methods
Dermatology
epidermis
Epidermis - cytology
Epidermis - physiology
Epidermis - ultrastructure
Epithelial Cells - cytology
Humans
in vivo model
Infant, Newborn
Keratinocytes - cytology
Keratinocytes - transplantation
Male
Medical sciences
Mice
Mice, SCID
Microscopy, Electron
Models, Animal
Mucous Membrane - cytology
rat trachea
Rats
Rats, Sprague-Dawley
Regeneration - physiology
Skin - cytology
skin regeneration
Stem Cells - cytology
Trachea - cytology
Trachea - transplantation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary::  Given that an important functional attribute of stem cells in vivo is their ability to sustain tissue regeneration, we set out to establish a simple and easy technique to assess this property from candidate populations of human keratinocyte stem cells in an in vivo setting. Keratinocytes were inoculated into devitalized rat tracheas and transplanted subcutaneously into SCID mice, and the epithelial lining regenerated characterized to establish the validity of this heterotypic model. Furthermore, the rate and quality of epidermal tissue reconstitution obtained from freshly isolated unfractionated vs. keratinocyte stem cell‐enriched populations was tested as a function of (a) cell numbers inoculated; and (b) the inclusion of irradiated support keratinocytes and dermal cells. Rapid and sustained epidermal tissue regeneration from small numbers of freshly isolated human keratinocyte stem cells validates the utilization of this simple and reliable model system to assay for enrichment of epidermal tissue‐reconstituting cells.
ISSN:0906-6705
1600-0625
DOI:10.1111/j.0906-6705.2005.00252.x