Long-chain inulin increases dendritic cells in the Peyer's patches and increases ex vivo cytokine secretion in the spleen and mesenteric lymph nodes of growing female rats, independent of zinc status

Prebiotics may increase Zn absorption, a mineral known to play a central role in the immune system. Zn-deficient states are characterised by suppressed immune function, while prebiotics may improve both gut and cell-mediated immunity. Our objective was to determine if inulin alters the number and pr...

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Bibliographic Details
Published in:British journal of nutrition 2009-06, Vol.101 (11), p.1653-1663
Main Authors: Ryz, Natasha R, Meddings, Jon B, Taylor, Carla G
Format: Article
Language:English
Subjects:
animal models
Animals
Biological and medical sciences
Cytokines
Cytokines - metabolism
Dendritic cells
Dendritic Cells - drug effects
Diet
dietary minerals
ex vivo cytokine secretion
Feeding. Feeding behavior
Female
females
Fundamental and applied biological sciences. Psychology
growth and development
Immune system
Immunoglobulin G - blood
Immunophenotyping
intestinal absorption
Intestinal Absorption - drug effects
Inulin
Inulin - pharmacology
long-chain inulin
lymph nodes
Lymph Nodes - drug effects
Lymph Nodes - immunology
Lymphatic system
Mesentery
nutritional status
Permeability - drug effects
Peyer's patches
Peyer's Patches - drug effects
Peyer's Patches - immunology
prebiotics
Rats
Rats, Sprague-Dawley
Rodents
Small intestine
spleen
Spleen - drug effects
Spleen - immunology
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Zinc
Zinc - blood
Zinc - deficiency
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Summary:Prebiotics may increase Zn absorption, a mineral known to play a central role in the immune system. Zn-deficient states are characterised by suppressed immune function, while prebiotics may improve both gut and cell-mediated immunity. Our objective was to determine if inulin alters the number and proportion of immune cells in the spleen, mesenteric lymph nodes (MLN) and Peyer's patches (PP), ex vivo cytokine secretion, intestinal permeability and Zn status in healthy as well as Zn-deficient rats. Weanling female rats were fed diets supplemented with 5 % cellulose (CEL) or 5 % inulin (PRE) for 4 weeks. The rats received the CEL or PRE diet ad libitum (ZN) or in restricted amounts (DR), or deficient in Zn (ZD) for another 4 weeks. The PRE-fed rats had a higher number and proportion of dendritic cells in PP, and greater ex vivo secretion of IL-2, IL-10 and interferon-γ from spleen and MLN cells compared with CEL-fed rats. PRE reduced the number and proportion of T cell receptor (TCR)-αβ+CD8+ cells in spleen and CD45RA+ cells in MLN compared with CEL. ZD rats had lower serum IgG2a and T cell numbers in MLN compared with ZN and DR rats. TCRγδ+ cell numbers in PP were higher in ZD-PRE rats compared with ZD-CEL rats. Femur Zn concentrations of DR-PRE rats were higher than those of DR-CEL rats. Intestinal permeability was unchanged. The higher proportion and number of dendritic cells in the PP of inulin-fed rats indicates a need for further research on how prebiotics and their metabolites affect immune function possibly through intestinal dendritic cells.
ISSN:0007-1145
1475-2662
DOI:10.1017/S000711450812342X