Optimising the tissue culture conditions for high efficiency transformation of indica rice

Establishment of high efficiency Agrobacterium-mediated transformation techniques has greatly accelerated the widespread application of transformation in japonica rice. However, transformation in indica rice remains difficult. In this study, we identify two new media for subculture and differentiati...

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Bibliographic Details
Published in:Plant cell reports 2005, Vol.23 (8), p.540-547
Main Authors: Lin, Y.J, Zhang, Q.F
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Biological and medical sciences
Biotechnology
Cell Culture Techniques - methods
Cell Differentiation
Cell Division
Cultivars
culture media
DNA, Bacterial - genetics
Efficiency
Eukaryotic cell cultures
field crops
Fundamental and applied biological sciences. Psychology
genetic transformation
genetic vectors
Kinetics
Methods. Procedures. Technologies
micropropagation
Miscellaneous
Oryza - cytology
Oryza - genetics
Oryza - growth & development
Oryza sativa
Plant cells and fungal cells
plant regeneration
Plants, Genetically Modified - cytology
Plants, Genetically Modified - growth & development
Rice
Species Specificity
tissue culture
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Summary:Establishment of high efficiency Agrobacterium-mediated transformation techniques has greatly accelerated the widespread application of transformation in japonica rice. However, transformation in indica rice remains difficult. In this study, we identify two new media for subculture and differentiation, the two major steps in the tissue culture process for transformation. These media were tested using four cultivars representing very different germplasms of indica rice. The results show that the new media significantly improved the growth rate and quality of the calli, and also increased the differentiation rate for all four cultivars tested. Use of these modified media in transformation experiments also greatly improved the transformation efficiency of all four indica cultivars.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-004-0843-6